Arsenic-related Bowen's disease and paraquat-related skin cancerous lesions show no detectable ras and p53 gene alterations
Journal
Cancer Letters
Journal Volume
86
Journal Issue
1
Pages
59-65
Date Issued
1994-10-28
Author(s)
Abstract
We have investigated point mutations of condons 12, 13, and 61 in H-, K-, and N-ras oncogenes as well as p53 tumour suppressor gene exon 5 through exon 9 by PCR-SSCP analysis in 26 skin biopsy tissues from 16 arsenic-related Bowen's disease patients and 6 skin samples from 4 paraquat manufacturing workers. No mutation was found. These results are different from findings with UV associated skin cancers. Interestingly, a silent change at condon 27 of H-ras in one allele was detected in all 4 paraquat manufacturing workers and in 2 of 16 arsenic-related Bowen's disease patients. It is likely that the molecular mechanisms involved in arsenic and paraquat induced skin cancers differ from sunlight-related skin maligancies. ? 1994.
Subjects
Bowen's disease; p53; PCR-SSCP; ras
SDGs
Other Subjects
arsenic; paraquat; protein p53; article; bowen disease; cancer genetics; chemical carcinogenesis; clinical article; controlled study; exon; human; human tissue; industrial worker; oncogene h ras; oncogene k ras; oncogene ras; point mutation; polymerase chain reaction; priority journal; single strand conformation polymorphism; skin biopsy; skin cancer; tumor suppressor gene; Arsenic; Base Sequence; Biopsy; Bowen's Disease; Codon; Genes, p53; Genes, ras; Human; Molecular Sequence Data; Occupational Diseases; Paraquat; Point Mutation; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Polymorphism, Single-Stranded Conformational; Skin Neoplasms; Support, Non-U.S. Gov't
Type
journal article