Utilizing nonlinear optical microscopy to diagnose human bladder tumor and the development of early cancer imaging techniques in nude mice in vivo
Date Issued
2005
Date
2005
Author(s)
Wang, Chun-Chin
DOI
zh-TW
Abstract
Tumor is a major disease in Taiwan and the development of minimally invasive techniques to diagnose bladder tumor will be of tremendous values for cancer diagnosis. In this investigation, we used nonlinear optical microscopy to image and analyze normal and cancerous bladder tissues. In order to monitor the early development of cancer cells, we also implemented a skin-fold chamber system for high resolution observation in vivo.
Our results show that a combination of second harmonic generation and two-photon autofluorescence imaging can be used to acquire molecular and structural information in distinguishing bladder carcinoma from normal tissues. With the application of ASI ( Autofluorescence versus Second harmonic generation Index ), we can obtain the qualitative and quantitative information for distinguishing normal and cancerous bladder tissues.
Utilizing the variation of autofluorescence and second harmonic generation signal, we can also visualize the interaction between squamous cells and connective tissue in vivo.
Subjects
非線性光學
雙光子
二倍頻
癌症
活體
nonlinear optics
two-photon
second harmonic generation
cancer
in vivo
SDGs
Type
thesis
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