https://scholars.lib.ntu.edu.tw/handle/123456789/321869
標題: | Inhibition of allergen-induced airway inflammation and hyperreactivity by recombinant lactic-acid bacteria | 作者: | YUH-CHYANG CHARNG Lin, C.-C. Hsu, C.-H. |
關鍵字: | Airway hyperreactivity; Allergy; Immunotherapy; Lactic-acid bacteria | 公開日期: | 2006 | 卷: | 24 | 期: | 33-34 | 起(迄)頁: | 5931-5936 | 來源出版物: | Vaccine | 摘要: | Recombinant lactic-acid bacteria (LAB) are able to inhibit allergen-specific T-cell responses. In this study, we examined whether oral feeding of recombinant LAB was able to suppress allergen-induced airway inflammation and hyperreactivity (AHR) in a murine model. Animals were intraperitoneally sensitized with Dermatophagoides pteronyssinus group-5 allergen (Der p 5) and orally treated with recombinant LAB containing a plasmid-encoded Der p 5 gene or placebo on day 7 and day 14 for three days consecutively. Twenty-one days after sensitization, mice underwent inhalational challenging. Der p 5-specific immunological responses including changes to specific immunoglobulin G and E (IgE) levels, the presence of cells in the bronchoalveolar lavage fluid (BALF), and AHR were assessed following this inhalational challenge. We demonstrated that oral feeding of recombinant LAB could significantly decrease the synthesis of Der p 5-specific IgE, and AHR. Furthermore, following such treatment, we also noted that both neutrophils and eosinophils had infiltrated the BALF to a significantly lower extent, when compared to the vehicle-treated group. Neither recombinant allergen nor LAB alone was able to suppress allergen-induced immune responses. Our findings suggest that treatment with recombinant LAB at a low dose can suppress allergen-induced airway allergic inflammation, this providing a basis for developing a novel therapeutic method for allergic airway diseases. ? 2006. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-33746224290&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/321869 |
DOI: | 10.1016/j.vaccine.2005.07.107 | SDG/關鍵字: | allergen; immunoglobulin E; immunoglobulin G; placebo; plasmid vector; recombinant lactic acid bacterium vaccine; recombinant vaccine; unclassified drug; airway constriction; allergic asthma; animal cell; animal experiment; animal model; article; bronchus hyperreactivity; comparative study; controlled study; Dermatophagoides pteronyssinus; drug inhibition; eosinophil; female; foraging; immune response; immunoglobulin blood level; immunoglobulin production; lactic acid bacterium; low drug dose; lung lavage; mouse; neutrophil chemotaxis; nonhuman; priority journal; provocation test; sensitization; treatment response; Administration, Oral; Animals; Antigens, Dermatophagoides; Bronchial Hyperreactivity; Bronchoalveolar Lavage Fluid; Desensitization, Immunologic; Enzyme-Linked Immunosorbent Assay; Female; Immunoglobulin E; Immunoglobulin G; Lactobacillus acidophilus; Mice; Probiotics; Respiratory Hypersensitivity |
顯示於: | 農藝學系 |
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