Ultrasensitive detection and characterization of posttranslational modifications using surface-enhanced Raman spectroscopy
Journal
Analytical Chemistry
Journal Volume
78
Journal Issue
11
Pages
3543-3550
Date Issued
2006-06
Author(s)
Sundararajan N.
Mao D.
Chan S.
Koo T.-W.
Su X.
Sun L.
Zhang J.
Yamakawa M.
Gafken P.R.
Randolph T.
McLerran D.
Feng Z.
Berlin A.A.
Roth M.B.
Abstract
Posttranslational modification (PTM) of proteins is likely to be the most common mechanism of altering the expression of genetic information. It is essential to characterize PTMs to establish a complete understanding of the activities of proteins. Here, we present a sensitive detection method using surface-enhanced Raman spectroscopy (SERS) that can detect PTMs from as little as zeptomoles of peptide. We demonstrate, using model peptides, the ability of SERS to detect a variety of protein modifications, such as acetylation, trimethylation, phosphorylation, and ubiquitination. In addition, we show the capability to obtain positional information for modifications such as trimethylation and phosphorylation using SERS and wavelet decomposition data analysis techniques. We further show that it is possible to apply SERS to detect PTMs from biological samples such as histones. We envision that this detection method might be a valuable technique that is complementary to mass spectrometry in obtaining orthogonal chemical and modification-specific information from biological samples at sensitive levels. © 2006 American Chemical Society.
Other Subjects
Genetic information; Phosphorylation; Posttranslational modification (PTM); Trimethylation; Ubiquitination; Wavelet decomposition data analysis techniques; Acetylation; Chemical modification; Data reduction; Mass spectrometry; Polypeptides; Raman spectroscopy; Proteins; peptide; acetylation; analytic method; article; decomposition; gene expression; methylation; protein phosphorylation; protein processing; Raman spectrometry; surface enhanced raman spectroscopy; ubiquitination; wavelet decomposition analysis; Acetylation; Amino Acid Sequence; Animals; Cattle; Histones; Methylation; Phosphorylation; Protein Processing, Post-Translational; Sensitivity and Specificity; Spectrum Analysis, Raman; Surface Properties; Thymus Gland
Type
journal article