https://scholars.lib.ntu.edu.tw/handle/123456789/328258
標題: | Characterization of retrovirus-based reporter viruses pseudotyped with the precursor membrane and envelope glycoproteins of four serotypes of dengue viruses | 作者: | Hu, Hsien-Ping Hsieh, Szu-Chia King, Chwan-Chuen Wang, Wei-Kung |
關鍵字: | Dengue virus; Pseudotype reporter virus | 公開日期: | 2007 | 卷: | 368 | 期: | 2 | 起(迄)頁: | 376-387 | 來源出版物: | Virology | 摘要: | In this study, we successfully established retrovirus-based reporter viruses pseudotyped with the precursor membrane and envelope (PrM/E) proteins of each of the four serotypes of dengue viruses, which caused the most important arboviral diseases in this century. Co-sedimentation of the dengue E protein and HIV-1 core proteins by sucrose gradient analysis of the pseudotype reporter virus of dengue virus type 2, D2(HIVluc), and detection of HIV-1 core proteins by immunoprecipitation with anti-E monoclonal antibody suggested that dengue viral proteins were incorporated into the pseudotype viral particles. The infectivity in target cells, as assessed by the luciferase activity, can be inhibited by the lysosomotropic agents, suggesting a pH-dependent mechanism of entry. Amino acid substitutions of the leucine at position 107, a critical residue at the fusion loop of E protein, with lysine resulted in severe impairment in infectivity, suggesting that entry of the pseudotype reporter virus is mediated through the fusogenic properties of E protein. With more and more dengue viral sequences available from different outbreaks worldwide, this sensitive and convenient tool has the potential to facilitate molecular characterization of the PrM/E proteins of dengue field isolates. ? 2007 Elsevier Inc. All rights reserved. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-35548932391&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/328258 |
DOI: | 10.1016/j.virol.2007.06.026 | SDG/關鍵字: | envelope protein; glycoprotein; leucine; luciferase; virus protein; amino acid substitution; article; controlled study; Dengue virus; enzyme activity; human; human cell; Human immunodeficiency virus 1; immunoprecipitation; nonhuman; pH; priority journal; Retrovirus; serotype; sucrose gradient; virus particle; Cell Line; Dengue Virus; Genes, Reporter; Genome, Viral; Hela Cells; HIV-1; Humans; K562 Cells; Luciferases; Mutagenesis, Site-Directed; Serotyping; Viral Envelope Proteins; Virion; Virology; Dengue virus; Dengue virus group; Dengue virus type 2; Human immunodeficiency virus 1 |
顯示於: | 流行病學與預防醫學研究所 |
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