The diabetogenic effects of the combination of humic acid and arsenic: In vitro and in vivo studies
Journal
Toxicology Letters
Journal Volume
172
Journal Issue
3
Pages
91-105
Date Issued
2007
Author(s)
Abstract
Black foot disease (BFD) is a peripheral arterial occlusive disease found among the inhabitants of the southwest coast of Taiwan. Moreover, within the BFD-endemic areas, diabetes mellitus occur at significantly higher rates than in other areas of Taiwan. A high concentration of humic acid (HA), and arsenic (As) are present in the artesian well water from BFD-endemic area. The aim of this paper is to study the diabetogenic effect of the combination of HA and AS. Treatment of HIT-T15 cells with HA, As, or both of them resulted loss of cell viability, apoptosis, depletion of ATP, increment of oxidative stress, activation of caspase 3, and dysfunction of insulin secretion. In addition, the plasma insulin of ICR mice, which were exposed to HA and As in drinking water for 12 weeks, was decreased in the 5, 7, and 12 weeks, and increased at early stage of exposure (3 weeks). The results reported herein reveal that HA and As exert HIT-T15 cell dysfunction and inhibited insulin secretive effects. In addition, the sub-acute peri-pancreatitis and islet damage caused by the infiltration of inflammatory cells after exposure of HA and As in drinking water for 5 weeks. Our study has important implications in the diabetogenic effect of the HA and AS which may be mediated by ROS and further information of the toxicity mechanisms will provide under our progressive studies. ? 2007 Elsevier Ireland Ltd. All rights reserved.
Subjects
Arsenic; Diabetogenic effect; HIT-T15 cells; Humic acid; Insulin; Oxidative stress
SDGs
Other Subjects
adenosine triphosphate; arsenic; caspase 3; humic acid; reactive oxygen metabolite; animal cell; animal experiment; animal model; animal tissue; apoptosis; article; cell disruption; cell survival; cell viability; controlled study; cytotoxicity; diabetes mellitus; diabetogenesis; endemic disease; enzyme activation; in vitro study; in vivo study; insulin blood level; insulin release; male; mouse; nonhuman; oxidative stress; pancreatitis; peripheral occlusive artery disease; priority journal; Adenosine Triphosphate; Animals; Apoptosis; Arsenic; Caspase 3; Cell Line; Cell Survival; Cricetinae; Diabetes Mellitus; Glutathione; Humic Substances; Insulin; Insulin-Secreting Cells; Male; Mice; Mice, Inbred ICR; Pancreas; Reactive Oxygen Species; Water Pollutants, Chemical; Mus
Type
journal article