https://scholars.lib.ntu.edu.tw/handle/123456789/328701
標題: | The diabetogenic effects of the combination of humic acid and arsenic: In vitro and in vivo studies | 作者: | SHING-HWA LIU | 關鍵字: | Arsenic; Diabetogenic effect; HIT-T15 cells; Humic acid; Insulin; Oxidative stress | 公開日期: | 2007 | 卷: | 172 | 期: | 3 | 起(迄)頁: | 91-105 | 來源出版物: | Toxicology Letters | 摘要: | Black foot disease (BFD) is a peripheral arterial occlusive disease found among the inhabitants of the southwest coast of Taiwan. Moreover, within the BFD-endemic areas, diabetes mellitus occur at significantly higher rates than in other areas of Taiwan. A high concentration of humic acid (HA), and arsenic (As) are present in the artesian well water from BFD-endemic area. The aim of this paper is to study the diabetogenic effect of the combination of HA and AS. Treatment of HIT-T15 cells with HA, As, or both of them resulted loss of cell viability, apoptosis, depletion of ATP, increment of oxidative stress, activation of caspase 3, and dysfunction of insulin secretion. In addition, the plasma insulin of ICR mice, which were exposed to HA and As in drinking water for 12 weeks, was decreased in the 5, 7, and 12 weeks, and increased at early stage of exposure (3 weeks). The results reported herein reveal that HA and As exert HIT-T15 cell dysfunction and inhibited insulin secretive effects. In addition, the sub-acute peri-pancreatitis and islet damage caused by the infiltration of inflammatory cells after exposure of HA and As in drinking water for 5 weeks. Our study has important implications in the diabetogenic effect of the HA and AS which may be mediated by ROS and further information of the toxicity mechanisms will provide under our progressive studies. ? 2007 Elsevier Ireland Ltd. All rights reserved. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-34547512317&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/328701 |
DOI: | 10.1016/j.toxlet.2007.05.008 | SDG/關鍵字: | adenosine triphosphate; arsenic; caspase 3; humic acid; reactive oxygen metabolite; animal cell; animal experiment; animal model; animal tissue; apoptosis; article; cell disruption; cell survival; cell viability; controlled study; cytotoxicity; diabetes mellitus; diabetogenesis; endemic disease; enzyme activation; in vitro study; in vivo study; insulin blood level; insulin release; male; mouse; nonhuman; oxidative stress; pancreatitis; peripheral occlusive artery disease; priority journal; Adenosine Triphosphate; Animals; Apoptosis; Arsenic; Caspase 3; Cell Line; Cell Survival; Cricetinae; Diabetes Mellitus; Glutathione; Humic Substances; Insulin; Insulin-Secreting Cells; Male; Mice; Mice, Inbred ICR; Pancreas; Reactive Oxygen Species; Water Pollutants, Chemical; Mus |
顯示於: | 毒理學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。