https://scholars.lib.ntu.edu.tw/handle/123456789/328760
標題: | Modulation of cytochrome P-450-dependent monooxygenases, glutathione and glutathione S-transferase in rat liver by geniposide from Gardenia jasminoides | 作者: | JAW-JOU KANG | 公開日期: | 1997 | 卷: | 35 | 期: | 10-11 | 起(迄)頁: | 957-965 | 來源出版物: | Food and Chemical Toxicology | 摘要: | Geniposide is an iridoid glycoside extracted from the fruits of Gardenia jasminoides, which are used as a food colorant and as a traditional Chinese medicine for treatment of hepatic and inflammatory diseases. The effects of geniposide and G. jasminoides fruit crude extract on liver cytochrome P-450 (P-450)-dependent monooxygenases, glutathione and glutathione S-transferase were investigated using rats treated orally with the iridoid glycoside (0.1 g/kg body weight/day) or the fruit crude extract (2 g/kg/day) for 4 days. The treatments decreased serum urea nitrogen level but increased liver to body weight ratio, total hepatic glutathione content and hepatic cytosolic glutathione S-transferase activity. Treatments with geniposide and G. jasminoides decreased P-450 content, benzo[a]pyrene hydroxylation, 7- ethoxycoumarin O-deethylation, and erythromycin N-demethylation activities in liver microsomes without affecting aniline hydroxylation activity. The natural products had no effect on glutathione content and monooxygenase activities in kidney microsomes. Immunoblotting analyses of liver microsomal proteins using mouse monoclonal antibody 2-13-1 to rat P4503A1/2 revealed that geniposide and G. jasminoides crude extract decreased the intensity of a P4503A-immunorelated protein. Protein blots probed with mouse monoclonal antibody 1-12-3 to rat P4501A1 and rabbit polyclonal antibody against human P4502E1 showed that both treatments had little or no effect on P4501A and 2E proteins. The present findings demonstrate that geniposide from G. jasminoides has the ability to inhibit a P4503A monooxygenase and increase glutathione content in rat liver. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-0031439453&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/328760 |
DOI: | 10.1016/S0278-6915(97)87265-1 | SDG/關鍵字: | 7 ethoxycoumarin; benzo[a]pyrene; cytochrome P450; erythromycin; Gardenia jasminoides extract; geniposide; glutathione; glutathione transferase; n demethylase; unspecific monooxygenase; animal experiment; animal tissue; article; controlled study; dose response; enzyme inhibition; fruit; hydroxylation; immunoblotting; liver; liver microsome metabolism; male; nonhuman; polyacrylamide gel electrophoresis; protein localization; rat; urea nitrogen blood level; Animalia; Gardenia angusta; Gardenia jasminoides; Oryctolagus cuniculus |
顯示於: | 毒理學研究所 |
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