https://scholars.lib.ntu.edu.tw/handle/123456789/328982
標題: | Recombinant porcine lactoferrin expressed in the milk of transgenic mice enhances offspring growth performance | 作者: | SHINN-CHIH WU Chen, H.-L. Yen, C.-C. Kuo, M.-F. Yang, T.-S. Wang, S.-R. Weng, C.-N. Chen, C.-M. Cheng, W.T.K. |
關鍵字: | Growth rate; Mammary gland; Porcine lactoferrin; Transgenic mice | 公開日期: | 2007 | 卷: | 55 | 期: | 12 | 起(迄)頁: | 4670-4677 | 來源出版物: | Journal of Agricultural and Food Chemistry | 摘要: | The European Commission has proposed a permanent ban on the use of antibiotics as an ingredient in animal feed to promote growth. Lactoferrin is a globular multifunctional protein that has been shown to play a role in iron absorption and to have antimicrobial and anti-inflammatory activities. Therefore, lactoferrin may serve as a nontherapeutic alternative to antibiotics in livestock husbandry. As a pilot study toward this goal, transgenic mice have been generated harboring a porcine lactoferrin (pLF) gene driven by the mammary gland-specific promoter of the bovine α-lactalbumin (αLA) gene. The αLA-pLF hybrid gene was confirmed to have been successfully integrated and transmitted stably through the germ-line in 9 (5 females and 4 males) of 14 transgenic founders. In the female progenies of six lines analyzed, the transgene copy numbers ranged from 1 to 20 with 1 - 4 integration sites. Significant levels of pLF protein in milk ranging from 40 to 106 μg/mL with physical characteristics similar to those of native pLF in sow's milk were achieved in three of the transgenic lines obtained. Tissue- and stage-specific pLF expressions were restricted to the mammary gland of the transgenic female mice during lactation. It was further demonstrated that the growth performance of animal pups is enhanced by directly feeding the genetically engineered milk containing enriched pLF protein in transgenic mice. Furthermore, this enhanced growth performance in suckling mice was proportional to the concentration of pLF present in milk. © 2007 American Chemical Society. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-34250744578&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/328982 |
DOI: | 10.1021/jf063759o | SDG/關鍵字: | lactalbumin; lactoferrin; animal; article; body weight; cattle; chemistry; duodenum; female; genetically modified food; genetics; intestine mucosa; lactation; microvillus; milk; molecular genetics; mouse; nucleotide sequence; physiology; polymerase chain reaction; promoter region; secretion; swine; transgenic mouse; ultrastructure; weight gain; Animals; Base Sequence; Body Weight; Cattle; Duodenum; Female; Food, Genetically Modified; Intestinal Mucosa; Lactalbumin; Lactation; Lactoferrin; Mice; Mice, Transgenic; Microvilli; Milk; Molecular Sequence Data; Polymerase Chain Reaction; Promoter Regions (Genetics); Swine; Weight Gain; Animalia; Bovinae; Mus; Mus musculus; Sus |
顯示於: | 動物科學技術學系 |
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J Agric. Food Chem. 2007 55 4670~4677.pdf | 675.52 kB | Adobe PDF | 檢視/開啟 |
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