Choline biosensor constructed with chitinous membrane from soldier crab and its application in measuring cholinesterase inhibitory activities

RICHIE CHEN; PO-CHUAN HSIEH; Matsumoto, Kiyoshi; Cheng, Tzong-Jih; Yuu, Gyokurin; Hsieh, B.-C.;Matsumoto, K.;Cheng, T.-J.;Yuu, G.;Chen, R.L.C.

doi:10.1016/j.jpba.2007.01.031

Choline biosensor constructed with chitinous membrane from soldier crab and its application in measuring cholinesterase inhibitory activities

Resource

Journal of Pharmaceutical and Biomedical Analysis 45 (4): 673-678

Journal

Journal of Pharmaceutical and Biomedical Analysis

Journal Volume

45

Journal Issue

4

Pages

673-678

Date Issued

2007

Author(s)

RICHIE CHEN

PO-CHUAN HSIEH

Matsumoto, Kiyoshi

Cheng, Tzong-Jih

Yuu, Gyokurin

DOI

10.1016/j.jpba.2007.01.031

URI

http://www.scopus.com/inward/record.url?eid=2-s2.0-35548954641&partnerID=MN8TOARS

http://scholars.lib.ntu.edu.tw/handle/123456789/329540

Abstract

An amperometric flow-injection choline biosensor was assembled utilizing natural chitinous membrane as the supporting material for biocatalyst immobilization, and the membrane was purified from Taiwanese soldier crab, Mictyris brevidactylus. The chitinous membrane (3 for 10.0 microM choline) and high reproducibility (CV<3% for 1.0mM choline, n=7). The system was proved to be useful in measuring cholinesterase inhibitory activities of synthetic chemicals or natural products.

Type

journal article

File(s)

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10.pdf

Size

979.38 KB

Format

Adobe PDF

Checksum

(MD5):93372bb73bf0ced995009618f6a5dcf8

Name

09.pdf

Size

23.95 KB

Format

Adobe PDF

Checksum

(MD5):4c883fc2622f75c87fc86b8d6ce04cee

Choline biosensor constructed with chitinous membrane from soldier crab and its application in measuring cholinesterase inhibitory activities

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