A 200-bp constructed inducible PR-1a promoter fusion to the Ac transposase gene drives higher transposition of a Ds element than the native PR-1a promoter fusion drives
Journal
Plant Science
Journal Volume
130
Journal Issue
1
Pages
73-86
Date Issued
1997
Author(s)
Abstract
An inducible transposon tagging technique in plants with a large genome was accessed. The open reading frame coding for the transposase gene of the maize transposon Activator (Ac) was expressed in transgenic tobacco plants under the control of the promoter of the inducible gene for pathogenesis- related protein 1a (PR-1a). Based on the native PR-1a promoter, several inducible promoters were constructed in order to induce variability of the Ac transposase expression level. Excision of a non-autonomous transposable element (Ds) from the chimeric β-glucuronidase gene construct was employed to analyze the induction of the Ac transposase by salicylic acid (SA). Applying the β-glucuronidase histochemical assay, Ds excision efficiency was determined in the regeneration calli after treatment of a tobacco leaf disc with SA. A 111-bp regulatory element, located between nucleotides - 699 to - 588 of the PR-1a promoter, was fused to the 89-bp CaMV35S core promoter to serve as the inducible promoter, PRΔD. When the PRΔD promoter was fused with the Ac transposase gene and induced by SA, it triggered a 26-fold higher Ds excision efficiency than the native PR-1a promoter fusion. Furthermore, Ds excision events occurred mainly in the regeneration calli at day 8 after induction with SA. Because Ds excision events could be induced in such a short period, an alternative gene tagging strategy is suggested.
SDGs
Type
journal article