https://scholars.lib.ntu.edu.tw/handle/123456789/332415
標題: | Sequence analysis of a duck picornavirus isolate indicates that it together with porcine enterovirus type 8 and simian picornavirus type 2 should be assigned to a new picornavirus genus | 作者: | Tseng, C.-H. HSIANG-JUNG TSAI |
關鍵字: | DPV; Duck picornavirus; Genome analysis; Picornaviridae | 公開日期: | 2007 | 卷: | 129 | 期: | 2 | 起(迄)頁: | 104-114 | 來源出版物: | Virus Research | 摘要: | In a 1990 outbreak, a virus isolated in Taiwan from the intestines of ducks showing signs of hepatitis was tentatively classified as a picornavirus on the basis of physical, chemical, and morphological characteristics. The virus was cloned and then found not to be type 1 duck hepatitis virus (DHV-1) or a new serotype of duck hepatitis virus (N-DHV) by serum neutralization. Complete genome sequencing indicated that the virus genome had 8351 nucleotides and the typical picornavirus genome organization (i.e., 5′ untranslated region (UTR)-L-P1 (VP 4-2-3-1)-P2 (2A-B-C)-P3 (3A-B-C-D)-3′ UTR-poly A). One open reading frame encoded 2521 amino acids, which makes this virus one of the largest picornaviruses, second only to equine rhinitis B virus of the genus Erbovirus. Its L protein was the largest within the family Picornaviridae (451 amino acids) and suspected to be a trypsin-like protease. The 235-nucleotide 3′ UTR region was of intermediate size, quite long compared to other picornaviruses but shorter than other picornaviruses of duck-origin (DHV-1 and N-DHV) and had four regions of secondary structure. The 2A protein was composed of only 12 amino acids, which is the shortest of any member of the family Picornaviridae. Phylogenetic analysis of the polyprotein and 3D sequences indicated that this virus (named duck picornavirus [DPV]) together with porcine enterovirus type 8 virus and several simian picornaviruses form a distinct branch of the family Picornaviridae and should be assigned to a new picornavirus genus. ? 2007 Elsevier B.V. All rights reserved. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-35348875388&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/332415 |
DOI: | 10.1016/j.virusres.2007.06.023 | SDG/關鍵字: | dihydrolipoamide dehydrogenase; virus protein; 3' untranslated region; amino acid sequence; animal cell; article; controlled study; Enterovirus; hepatitis; hepatitis virus; nonhuman; nucleotide sequence; open reading frame; phylogeny; Picornavirus; priority journal; sequence analysis; Taiwan; untranslated region; virus gene; virus genome; virus neutralization; 3' Untranslated Regions; 5' Untranslated Regions; Amino Acid Sequence; Animals; Base Sequence; Ducks; Enteroviruses, Porcine; Genome, Viral; Molecular Sequence Data; Nucleic Acid Conformation; Phylogeny; Picornaviridae; Sequence Alignment; Taiwan; Viral Proteins; Anas; Duck hepatitis B virus; Enterovirus; Equine rhinitis B virus; Erbovirus; Picornaviridae; Simiae; Sus |
顯示於: | 獸醫學系 |
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