https://scholars.lib.ntu.edu.tw/handle/123456789/335268
標題: | Comparison of immunohistochemical and fluorescence in situ hybridization assessment for HER-2/neu status in Taiwanese breast cancer patients | 作者: | MING CHEN | 關鍵字: | Breast cancer; Fluorescence in situ hybridization; HER-2; Immunohistochemistry; Topoisomerase IIα | 公開日期: | 2007 | 卷: | 46 | 期: | 2 | 起(迄)頁: | 146-151 | 來源出版物: | Taiwanese Journal of Obstetrics and Gynecology | 摘要: | Objective: Accurate diagnostic assessment of human epidermal growth factor receptor-2 (HER-2) is essential and a prerequisite for appropriate application of the humanized anti-HER-2 monoclonal antibody trastuzumab (Herceptin) to the treatment of patients with breast cancer. Immunohistochemistry (IHC) is the most widely applicable diagnostic modality in studying HER-2 status. Fluorescence in situ hybridization (FISH) is also recognized as a modality in cases with an equivocal IHC status (score, 2+). Some authors claimed that FISH alone is sufficient. The aim of this study was to correlate the test results of IHC and FISH for HER-2 gene amplification in breast cancer patients. FISH for topoisomerase IIα (TOP2A) was also studied to see if deletion or amplification of TOP2A has any supplementary role to HER-2, FISH and IHC. Materials and Methods: Assessment of HER-2 gene amplification and TOP2A gene amplification/deletion was made by FISH analysis using the LSI TOP2A/HER-2/CEP 17 multicolor probe or the LSI HER-2/CEP dual color probe (Vysis, Downers Grove, IL, USA) in formalin-fixed and paraffin-embedded tissue sections of 54 breast cancer patients who were grouped into stages 1+, 2+ or 3+ based on IHC (HercepTest; DakoCytomation, Carpinteria, CA, USA) observations. Results: None of IHC 1+ breast tumors was HER-2 FISH positive, but three of 18 (17%) IHC 3+ tumors were HER-2 FISH negative. Overall, 53% of the IHC 2+ and 83% of the IHC 3+ cases were HER-2 FISH positive. Only one case with IHC 3+ tumor that was HER-2 FISH positive was found to have TOP2A amplification (> 2.0) and no IHC 2+ cases were found to have TOP2A amplification. There were no cases with TOP2A deletion (< 0.8) in our whole series. There were also no cases of HER-2 FISH negative tumors, but IHC scored as 2+ or 3+(0 of 10), to be found with TOP2A amplification. The discordance rates by IHC were high (46.7% in IHC 2+, 16.7% in IHC 3+, 30.3% overall in IHC 2+ or 3+). On the contrary, the discordance rates were zero if by FISH. Conclusion: The current algorithm to use HER-2 FISH as a supplementary role to IHC HercepTest 2+ may need some modifications according to the local setting. TOP2A FISH adds little value to HER-2 FISH and IHC staining in our study. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-34547795209&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/335268 |
DOI: | 10.1016/S1028-4559(07)60008-4 | SDG/關鍵字: | DNA topoisomerase (ATP hydrolysing); epidermal growth factor receptor 2; trastuzumab; article; breast biopsy; breast cancer; breast tumor; cancer patient; cancer staging; controlled study; fluorescence in situ hybridization; gene amplification; gene deletion; gene overexpression; gene probe; human; human tissue; immunohistochemistry; major clinical study |
顯示於: | 醫學系 |
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