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  4. Effects of psoralen plus ultraviolet A irradiation on cultured epidermal cells in vitro and patients with vitiligo in vivo
 
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Effects of psoralen plus ultraviolet A irradiation on cultured epidermal cells in vitro and patients with vitiligo in vivo

Journal
British Journal of Dermatology
Journal Volume
156
Journal Issue
1
Pages
122-129
Date Issued
2007
Author(s)
LI-FANG WANG  
DOI
10.1111/j.1365-2133.2006.07584.x
URI
http://www.scopus.com/inward/record.url?eid=2-s2.0-33845545980&partnerID=MN8TOARS
http://scholars.lib.ntu.edu.tw/handle/123456789/335341
Abstract
Background: Both psoralen plus ultraviolet (UV) A (PUVA) and narrowband UVB (NB-UVB) irradiation are effective treatments for vitiligo vulgaris. However, the mechanisms of PUVA and NB-UVB in repigmentation are not thoroughly clarified. Our previous results showed that NB-UVB irradiation directly promotes melanocyte (MC) migration and stimulates MC proliferation via keratinocytes (KCs). Objectives: In the present study, we used NB-UVB as a reference for comparison to investigate the immediate effects of PUVA on MC proliferation and migration. Methods: Cultured MCs and KCs were treated with PUVA or irradiated with NB-UVB. The direct impact of PUVA treatment on MCs was assessed in terms of its effect on MC proliferation and migration. The indirect effect of PUVA treatment and NB-UVB irradiation on MC proliferation via KCs was also investigated. The activities of matrix metalloproteinase (MMP)-2 and MMP-9, known for their influence on cell migration, were evaluated in the PUVA-treated MC and KC supernatants. The concentrations of MC mitogens/growth factors in the PUVA-treated KC supernatants were also determined. In addition, the serum levels of MC mitogens/growth factors in healthy controls, in patients with active vitiligo and in patients with repigmenting vitiligo after PUVA treatment were determined to elucidate the mechanisms of how PUVA induces vitiligo repigmentation in vivo. Results: Our results demonstrated that PUVA treatment did not significantly stimulate the release of MC mitogens/growth factors from KCs. The migration of MCs was also not enhanced after PUVA treatment. The expression of MMP-2 activity in supernatants derived from PUVA-treated MCs was significantly increased as compared with the control group. However, neither MMP-2 nor MMP-9 activity in KC supernatants was stimulated by PUVA treatment. In contrast to NB-UVB, immediate effects of PUVA on MC proliferation and migration were not observed in this study. Sera from patients with repigmenting vitiligo after PUVA treatment contained higher levels of basic fibroblast growth factor, stem cell factor and hepatocyte growth factor as compared with healthy controls and patients with active vitiligo. Conclusions: Our results indicate that in addition to immune suppression, PUVA treatment creates a favourable milieu for promoting the growth of MCs in patients with vitiligo instead of directly stimulating the regrowth of MCs. Based on our results, we propose that in the active stage of vitiligo, PUVA treatment is the therapy of choice to slow down the destruction of MCs and to create a favourable environment for MCs to survive. In the stable stage of vitiligo, NB-UVB irradiation should be used to stimulate the proliferation and migration of MCs directly. ? 2007 The Authors.
Subjects
Keratinocyte; Melanocyte; Psoralen plus ultraviolet A; Repigmentation; Vitiligo
SDGs

[SDGs]SDG3

Other Subjects
basic fibroblast growth factor; gelatinase A; gelatinase B; growth factor; mitogenic agent; psoralen; scatter factor; stem cell factor; adolescent; adult; article; cell culture; cell migration; cell proliferation; clinical article; controlled study; drug effect; enzyme activity; enzyme release; epidermis cell; female; human; human cell; in vitro study; in vivo culture; keratinocyte; male; priority journal; PUVA; school child; ultraviolet A radiation; ultraviolet B radiation; vitiligo; Cell Movement; Cell Proliferation; Cells, Cultured; Ficusin; Humans; Keratinocytes; Melanocytes; Photosensitizing Agents; PUVA Therapy; Vitiligo
Type
journal article

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