Repository logo
  • English
  • 中文
Log In
Have you forgotten your password?
  1. Home
  2. College of Bioresources and Agriculture / 生物資源暨農學院
  3. Food Science and Technology / 食品科技研究所
  4. Involvement of activating transcription factors JNK, NF-κB, and AP-1 in apoptosis induced by pyrrolidine dithiocarbamate/Cu complex
 
  • Details

Involvement of activating transcription factors JNK, NF-κB, and AP-1 in apoptosis induced by pyrrolidine dithiocarbamate/Cu complex

Journal
European Journal of Pharmacology
Journal Volume
594
Journal Issue
1-3
Pages
9-17
Date Issued
2008
Author(s)
Chen, S.-H.
Lin, J.-K.
Liang, Y.-C.
MIN-HSIUNG PAN  
SHING-HWA LIU  
Lin-Shiau, S.-Y.
DOI
10.1016/j.ejphar.2008.07.024
URI
http://www.scopus.com/inward/record.url?eid=2-s2.0-50549096389&partnerID=MN8TOARS
http://scholars.lib.ntu.edu.tw/handle/123456789/338478
Abstract
Pyrrolidine dithiocarbamate (PDTC) is a metal chelator. Biologically, slight toxic affects EC50, 100 ± 5.9?μM are observed when added to cultured HL-60 cells. CuCl2 at a physiological concentration (1?μM), but not FeCl2, Pb potentiated the cytotoxic effect of PDTC by 700 fold (EC50, 0.14 ± 0.02?μM). Furthermore, results indicated that the PDTC/Cu complex induced an apoptotic process, evidenced by apoptotic bodies, DNA ladder and hypodiploidy cells. Additional studies showed that PDTC/Cu complex significantly decreased mitochondrial membrane potential, increased cytochrome c release, and reactive oxygen species production, and depleted reduced non-protein thiols in a time-dependent manner. Following oxidative stress, the PDTC/Cu complex sequentially activated JNK, NF-κB and AP-1 signaling pathways while IκB kinase activity was enhanced. The apoptotic process was eventually induced by caspase 3 activation and PARP degradation. The non-permeable copper-specific chelator-bathocuproine disulfonate (BCPS) and vitamin C were able to inhibit apoptosis and the elevation of intracellular Cu. Based on these findings; we conclude that PDTC/Cu complex-induced apoptosis is mediated by activation of JNK, NF-κB, AP-1 and caspase 3. Due to its high potency, PDTC may be useful as a therapeutic anti-cancer drug. ? 2008.
Subjects
AP-1; Apoptosis; BCPS; Caspase; Copper; JNK; NF-κB; Oxidative stress; PDTC
SDGs

[SDGs]SDG3

Other Subjects
ascorbic acid; bathocuproine disulfonate; chelating agent; copper; copper chloride; cytochrome c; I kappa B kinase; immunoglobulin enhancer binding protein; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase; pyrrolidine dithiocarbamate; reactive oxygen metabolite; stress activated protein kinase; transcription factor AP 1; unclassified drug; apoptosis; article; cell culture; cell level; cell strain HL 60; concentration response; controlled study; culture medium; drug cytotoxicity; drug effect; drug mechanism; drug potency; enzyme activation; enzyme activity; enzyme degradation; enzyme release; human; human cell; mitochondrial membrane potential; priority journal; signal transduction; time; Apoptosis; Caspase 3; Cell Survival; Copper; Cytochromes c; DNA Fragmentation; Electrophoretic Mobility Shift Assay; Flow Cytometry; Free Radicals; HL-60 Cells; Humans; I-kappa B Kinase; JNK Mitogen-Activated Protein Kinases; Membrane Potentials; Mitochondrial Membranes; NF-kappa B; Poly(ADP-ribose) Polymerases; Pyrrolidines; Spectrophotometry, Atomic; Sulfhydryl Compounds; Thiocarbamates; Transcription Factor AP-1
Type
journal article

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

  • 請確認所上傳的全文是原創的內容,若該文件包含部分內容的版權非匯入者所有,或由第三方贊助與合作完成,請確認該版權所有者及第三方同意提供此授權。
    Please represent that the submission is your original work, and that you have the right to grant the rights to upload.
  • 若欲上傳已出版的全文電子檔,可使用Open policy finder網站查詢,以確認出版單位之版權政策。
    Please use Open policy finder to find a summary of permissions that are normally given as part of each publisher's copyright transfer agreement.
  • 網站簡介 (Quickstart Guide)
  • 使用手冊 (Instruction Manual)
  • 線上預約服務 (Booking Service)
  • 方案一:臺灣大學計算機中心帳號登入
    (With C&INC Email Account)
  • 方案二:ORCID帳號登入 (With ORCID)
  • 方案一:定期更新ORCID者,以ID匯入 (Search for identifier (ORCID))
  • 方案二:自行建檔 (Default mode Submission)
  • 方案三:學科館員協助匯入 (Email worklist to subject librarians)

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science