Modularly assembled magnetite nanoparticles enhance in vivo targeting for magnetic resonance cancer imaging
Journal
Bioconjugate Chemistry
Journal Volume
19
Journal Issue
10
Pages
1972 - 1979
Date Issued
2008-10
Author(s)
Ping-Ching Wu
Chia-Hao Su
Fong-Yu Cheng
Jun-Cheng Weng
Tsung-Lin Tsai
Chen-Sheng Yeh
Wu-Chou Su
Jih Ru Hwu
Yonhua Tzeng
Dar-Bin Shieh
Abstract
Modularly assembled targeting nanoparticles were synthesized through self-assembly of targeting moieties on surfaces of functional nanoparticles. Specific molecular recognition of nickel nitrilotriacetate on Fe 3O4 nanoparticles with hexahistidine tag on RGD4C peptides results in precisely controlled orientation of the targeting peptides. Better selectivity of the self-assembled RGD4C-Fe3O4 nanoparticles targeting oral cancer cells than that achievable through a conventional chemical cross-link strategy was demonstrated by means of atomic absorption spectrometry (AAS). An oral cancer hamster model was applied to reveal specific in vivo targeting and MR molecular imaging contrast in cancer lesions expressing αvβ3 integrin. Both AAS and MRI revealed that the self-assembled nanoparticles improved the targeting efficiency and reduced the hepatic uptake as compared with the conventional chemical cross-link particles. We investigated the biosafety, biodistribution, and kinetics of the nanoparticles and found that the nanoparticles were significantly cleared from the liver and kidneys after one week. By recombining the desired targeting moiety and various functional nanoparticles through self-assembly, this new modularly designed platform has the capability of enhancing the efficiency of targeted diagnosis and therapies for a wide spectrum of biomedical applications. © 2008 American Chemical Society.
SDGs
Other Subjects
Absorption spectroscopy; Atomic absorption spectrometry; Atoms; Diseases; Efficiency; Magnetite nanoparticles; Molecular imaging; Nanomagnetics; Peptides; Synthesis (chemical); Atomic-absorption spectrometry; Cancer imaging; Controlled orientation; Functional nanoparticles; Hexahistidine tag; In-vivo; Nanoparticle targeting; Synthesised; Targeting peptides; Vivo targeting; Magnetite; arginylglycylaspartic acid; magnetite; nanoparticle; vitronectin receptor; animal cell; animal experiment; animal model; article; atomic absorption spectrometry; biocompatibility; contrast enhancement; controlled study; drug conjugation; drug cytotoxicity; drug selectivity; drug targeting; drug uptake; histopathology; human; human cell; liver; male; molecular imaging; mouse; mouth cancer; nonhuman; nuclear magnetic resonance imaging; Syrian hamster; Cricetinae
Type
journal article