https://scholars.lib.ntu.edu.tw/handle/123456789/342634
標題: | Modularly assembled magnetite nanoparticles enhance in vivo targeting for magnetic resonance cancer imaging | 作者: | Ping-Ching Wu Chia-Hao Su Fong-Yu Cheng Jun-Cheng Weng JYH-HORNG CHEN Tsung-Lin Tsai Chen-Sheng Yeh Wu-Chou Su Jih Ru Hwu Yonhua Tzeng Dar-Bin Shieh |
公開日期: | 十月-2008 | 卷: | 19 | 期: | 10 | 起(迄)頁: | 1972 - 1979 | 來源出版物: | Bioconjugate Chemistry | 摘要: | Modularly assembled targeting nanoparticles were synthesized through self-assembly of targeting moieties on surfaces of functional nanoparticles. Specific molecular recognition of nickel nitrilotriacetate on Fe 3O4 nanoparticles with hexahistidine tag on RGD4C peptides results in precisely controlled orientation of the targeting peptides. Better selectivity of the self-assembled RGD4C-Fe3O4 nanoparticles targeting oral cancer cells than that achievable through a conventional chemical cross-link strategy was demonstrated by means of atomic absorption spectrometry (AAS). An oral cancer hamster model was applied to reveal specific in vivo targeting and MR molecular imaging contrast in cancer lesions expressing αvβ3 integrin. Both AAS and MRI revealed that the self-assembled nanoparticles improved the targeting efficiency and reduced the hepatic uptake as compared with the conventional chemical cross-link particles. We investigated the biosafety, biodistribution, and kinetics of the nanoparticles and found that the nanoparticles were significantly cleared from the liver and kidneys after one week. By recombining the desired targeting moiety and various functional nanoparticles through self-assembly, this new modularly designed platform has the capability of enhancing the efficiency of targeted diagnosis and therapies for a wide spectrum of biomedical applications. © 2008 American Chemical Society. |
URI: | http://scholars.lib.ntu.edu.tw/handle/123456789/342634 https://www.scopus.com/inward/record.uri?eid=2-s2.0-55249108013&doi=10.1021%2fbc800092w&partnerID=40&md5=2946b93bd75430f8edd41eecdade04ee |
ISSN: | 10431802 | DOI: | 10.1021/bc800092w | SDG/關鍵字: | Absorption spectroscopy; Atomic absorption spectrometry; Atoms; Diseases; Efficiency; Magnetite nanoparticles; Molecular imaging; Nanomagnetics; Peptides; Synthesis (chemical); Atomic-absorption spectrometry; Cancer imaging; Controlled orientation; Functional nanoparticles; Hexahistidine tag; In-vivo; Nanoparticle targeting; Synthesised; Targeting peptides; Vivo targeting; Magnetite; arginylglycylaspartic acid; magnetite; nanoparticle; vitronectin receptor; animal cell; animal experiment; animal model; article; atomic absorption spectrometry; biocompatibility; contrast enhancement; controlled study; drug conjugation; drug cytotoxicity; drug selectivity; drug targeting; drug uptake; histopathology; human; human cell; liver; male; molecular imaging; mouse; mouth cancer; nonhuman; nuclear magnetic resonance imaging; Syrian hamster; Cricetinae |
顯示於: | 電機工程學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。