https://scholars.lib.ntu.edu.tw/handle/123456789/346506
Title: | Epicutaneous administration of hapten through patch application augments TH2 responses which can downregulate the elicitation of murine contact hypersensitivity | Authors: | LI-FANG WANG | Keywords: | Contact hypersensitivity; Hapten; Patch application; T-cell deviation | Issue Date: | 1999 | Journal Volume: | 29 | Journal Issue: | 2 | Start page/Pages: | 271-279 | Source: | Clinical and Experimental Allergy | Abstract: | Background and objective: Allergic contact dermatitis and its animal model, contact hypersensitivity (CHS), have long been documented as type 1 T- cell-predominant immune responses. Although type 1/type 2 T-cell deviation has been repeatedly demonstrated to play an important role in many human diseases and their animal models, the potential of tilting type 1/type 2 T- cell differentiation of CHS by modulating the manner of administration and dosage of hapten remains unexplored. This study examined the effect of these two factors on type 1/type 2 balance of CHS. Methods: ELISA methods for detection of isotypes of hapten-specific antibodies and cytokine profiles of in vitro reactivation culture as well as ear-swelling assay were used to indicate type 1 or type 2 T-cell immune responses. Results: In this paper, it was demonstrated that dosage of hapten has no effect on the type 1/type 2 T- cell balance of CHS, whereas epicutaneous administration of hapten through patch application could tilt the type 1/type 2 balance to decrease type 1 and to augment type 2 T-cell responses. Patch application-induced modulation is still effective in ever-sensitized mice and the augmented type 2 T-cell responses are persistent and increase progressively in strength after repeated immunizations. Moreover, it was demonstrated that the augmented type 2 T-cell response can downregulate the elicitation of CHS. The major mediating cells of the enhanced type 2 T-cell responses were determined to be CD4+ T cells (TH2 cells). Conclusions: These data show that epicutaneous administration of hapten through patch application augments TH2 response which can downregulate the elicitation of murine CHS. This exploration may contribute to the understanding of regulatory mechanisms involved in contact allergy. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-0033010530&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/346506 |
DOI: | 10.1046/j.1365-2222.1999.00498.x | SDG/Keyword: | gamma interferon; hapten; hapten antibody; interleukin 4; picryl chloride; trinitrobenzenesulfonic acid; adoptive transfer; animal experiment; animal model; animal tissue; antibody detection; article; contact allergy; controlled study; cytokine release; dose response; down regulation; female; immune response; immunomodulation; lymphocyte differentiation; mouse; nonhuman; patch test; pathophysiology; priority journal; skin allergy; t lymphocyte subpopulation; Adoptive Transfer; Animals; Cytokines; Dermatitis, Allergic Contact; Down-Regulation; Enzyme-Linked Immunosorbent Assay; Female; Lymph Nodes; Mice; Mice, Inbred BALB C; Patch Tests; Picryl Chloride; Skin; Spleen; Th1 Cells; Th2 Cells |
Appears in Collections: | 分子醫學研究所 |
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