Localized deposition of chitosan as matrix for enzyme immobilization
Resource
Electroanalysis 21 (7): 804-810
Journal
Electroanalysis
Journal Volume
21
Journal Issue
7
Pages
804-810
Date Issued
2009
Author(s)
Abstract
Abstract A local electrodeposition method was developed for chitosan by exploiting a pH gradient between a macroscopic electrode (the support) and a much smaller counter electrode. The deposition was confined either by using the direct mode of scanning electrochemical microscopy (SECM) or by performing the deposition in channels of a microfluidic network. The roughness was characterized by noncontact scanning force microscopy. The availability of amino groups at the surface of the microstructures was visualized after labeling by confocal laser scanning microscopy. The enzyme glucose oxidase could be entrapped during the electrochemical deposition and showed activity as seen by SECM images.
SDGs
Type
journal article
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