https://scholars.lib.ntu.edu.tw/handle/123456789/348404
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Wu, S.-J. | en_US |
dc.contributor.author | Chang, S.-P. | en_US |
dc.contributor.author | Lin, D.-L. | en_US |
dc.contributor.author | Wang, S.-S. | en_US |
dc.contributor.author | Hou, F.-F. | en_US |
dc.contributor.author | Ng, L.-T. | en_US |
dc.contributor.author | LEAN-TEIK HUANG | en_US |
dc.creator | Wu, S.-J.;Chang, S.-P.;Lin, D.-L.;Wang, S.-S.;Hou, F.-F.;Ng, L.-T. | - |
dc.date.accessioned | 2018-09-10T07:31:24Z | - |
dc.date.available | 2018-09-10T07:31:24Z | - |
dc.date.issued | 2009 | - |
dc.identifier.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-67349115692&partnerID=MN8TOARS | - |
dc.identifier.uri | http://scholars.lib.ntu.edu.tw/handle/123456789/348404 | - |
dc.description.abstract | Physalis peruviana L. (PP) is a popular folk medicine used for treating cancer, leukemia, hepatitis, rheumatism and other diseases. In this study, our objectives were to examine the total flavonoid and phenol content of different PP extracts (aqueous: HWEPP; ethanolic: EEPP; supercritical carbon dioxide: SCEPP-0, SCEPP-4 and SCEPP-5) and their antiproliferative effects in human lung cancer H661 cells. Among all the extracts tested, results showed that SCEPP-5 possessed the highest total flavonoid (226.19 ± 4.15 mg/g) and phenol (100.82 ± 6.25 mg/g) contents. SCEPP-5 also demonstrated the most potent inhibitory effect on H661 cell proliferation. Using DNA ladder and flow cytometry analysis, SCEPP-5 effectively induced H661 cell apoptosis as demonstrated by the accumulation of Sub-G1 peak and fragmentation of DNA. SCEPP-5 not only induced cell cycle arrest at S phase, it also up-regulated the expression of pro-apoptotic protein (Bax) and down-regulated the inhibitor of apoptosis protein (IAP). Furthermore, the apoptotic induction in H661 cells was found to associate with an elevated p53 protein expression, cytochrome c release, caspase-3 activation and PARP cleavage. Taken together, these results conclude that SCEPP-5 induced cell cycle arrest at S phase, and its apoptotic induction could be mediated through the p53-dependent pathway and modification of Bax and XIAP proteins expression. The results have also provided important pharmacological backgrounds for the potential use of PP supercritical fluid extract as products for cancer prevention. ? 2009. | - |
dc.language | en | en |
dc.relation.ispartof | Food and Chemical Toxicology | en_US |
dc.source | AH | - |
dc.subject | Apoptosis; Cell cycle arrest; Physalis peruviana; Supercritical carbon dioxide extraction | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | alcohol; carbon dioxide; caspase 3; cytochrome c; DNA; flavonoid; hot water; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase; phenol; Physalis peruviana extract; plant extract; protein Bax; protein p53; unclassified drug; X linked inhibitor of apoptosis; antineoplastic activity; apoptosis; article; cancer cell culture; cancer prevention; cell cycle arrest; cell cycle S phase; concentration response; controlled study; DNA fragmentation; down regulation; drug cytotoxicity; drug effect; drug synthesis; enzyme activation; flow cytometry; human; human cell; lung cancer; Physalis; physalis peruviana; protein cleavage; protein expression; protein secretion; supercritical fluid; supercritical fluid extraction; upregulation; Antineoplastic Agents, Phytogenic; Apoptosis; Blotting, Western; Carbon Dioxide; Cell Cycle; Cell Line, Tumor; Cell Proliferation; Chromatography, Supercritical Fluid; DNA Fragmentation; Flavonoids; Gene Expression Regulation; Humans; Indicators and Reagents; Lung Neoplasms; Phenols; Physalis; Plant Extracts; Physalis peruviana | - |
dc.title | Supercritical carbon dioxide extract of Physalis peruviana induced cell cycle arrest and apoptosis in human lung cancer H661 cells | - |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.fct.2009.01.044 | - |
dc.relation.pages | 1132-1138 | - |
dc.relation.journalvolume | 47 | - |
dc.relation.journalissue | 6 | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
crisitem.author.dept | Agricultural Chemistry | - |
crisitem.author.orcid | 0000-0003-4064-7071 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
顯示於: | 農業化學系 |
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