|Title:||In vivo harmonic generation biopsy of human skin||Authors:||Chen, S.-Y.
|Keywords:||Dermatology; Harmonic generation microscopy; High penetrability; High resolution; Human skin; In vivo; Noninvasive||Issue Date:||2009||Journal Volume:||14||Journal Issue:||6||Source:||Journal of Biomedical Optics||Abstract:||
The ability to in vivo image deep tissues noninvasively with a high resolution is strongly required for optical virtual biopsy. Higher harmonic generation microscopy, combined with second- and third-harmonic generation microscopies, is applied to 17 Asian volunteers' forearm skin. After continuous observation for 30 min, no visible damage was found. Our study proves that harmonic generation biopsy (HGB) is able to satisfy the safety requirement and to provide high penetrability (?300 μm) and submicron resolution all at the same time and is a promising tool for future virtual biopsy of skin diseases. In contrast to a previous study on fixed human skin specimens, a much improved penetrability and much reduced resolution-degradation versus depth are found in this in vivo examination. ? 2009 Society of Photo-Optical Instrumentation Engineers.
|DOI:||10.1117/1.3269676||SDG/Keyword:||Harmonic generation microscopy; High penetrability; High resolution; Human skin; In vivo; Noninvasive; Biopsy; Dermatology; Harmonic analysis; Tissue; Virtual reality; Harmonic generation; acoustics; adult; biopsy; cytochemistry; cytology; diagnostic imaging; female; human; letter; male; methodology; microscopy; middle aged; pathology; skin; skin disease; Acoustics; Adult; Biopsy; Diagnostic Imaging; Female; Histocytochemistry; Humans; Male; Microscopy; Microscopy, Video; Middle Aged; Skin; Skin Diseases
|Appears in Collections:||電機工程學系|
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