Diosgenin attenuates allergen-induced intestinal inflammation and IgE production in a murine model of food allergy
Journal
Planta Medica
Journal Volume
75
Journal Issue
12
Pages
1300-1305
Date Issued
2009
Author(s)
Abstract
Diosgenin, the major sapogenin contained in the Chinese yam, has recently been shown to promote systemic T helper 1-type immunity in a murine model of airway hypersensitivity. In this study, we hypothesized that diosgenin might be effective in modulating food allergy. BALB/c mice were either left untreated (na?ve; NA) or administered daily with vehicle (VH; olive oil) and/or diosgenin (100 or 200mg/kg) by gavage throughout the experiment. Except for the NA group, the mice were sensitized with ovalbumin (OVA) and repeatedly challenged with intragastric OVA to induce intestinal allergic responses. Diosgenin demonstrated a suppressive effect on the intestinal inflammation, including the occurrence of diarrhea, the infiltration and degranulation of mast cells, and the presence of mucin-containing goblet cells in the duodenum. A protective effect by diosgenin on reducing the crypt depth of the intestine was also observed in OVA-sensitized and challenged mice. Furthermore, the serum production of OVA-specific IgE, and the total IgE was suppressed. In contrast, OVA-specific IgG2a was enhanced by diosgenin treatment in OVA-sensitized mice. These results demonstrated the in vivo anti-allergic activity of diosgenin, which is associated with the suppression of IgE production and mast cell infiltration and degranulation. ? Georg Thieme Verlag KG Stuttgart.
Subjects
Diosgenin; Food allergy; IgE; Mast cells; Ovalbumin
SDGs
Other Subjects
diosgenin; food allergen; immunoglobulin E; immunoglobulin G2a; ovalbumin; allergic reaction; animal experiment; animal model; article; Bagg albino mouse; cell infiltration; controlled study; degranulation; diarrhea; enteritis; feeding; food allergy; goblet cell; immunoglobulin production; immunomodulation; in vivo study; male; mast cell; mouse; nonhuman; Allergens; Animals; Diosgenin; Disease Models, Animal; Food Hypersensitivity; Immunoglobulin E; Male; Mice; Mice, Inbred BALB C; Dioscorea polystachya; Murinae; Mus
Type
journal article