https://scholars.lib.ntu.edu.tw/handle/123456789/355322
DC 欄位 | 值 | 語言 |
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dc.contributor.author | SHING-HWA LIU | en |
dc.contributor.author | Liu, Shing Hwa | en |
dc.contributor.author | Shen, Chin Chang | en |
dc.contributor.author | Yi, Yu Chiao | en |
dc.contributor.author | Tsai, Jaw Ji | en |
dc.contributor.author | Wang, Chih Chien | en |
dc.contributor.author | Chueh, Ju Ting | en |
dc.contributor.author | Lin, Keh Liang | en |
dc.contributor.author | Lee, Tso Ching | en |
dc.contributor.author | Pan, Hung Chuan | en |
dc.contributor.author | Sheu, Meei Ling | en |
dc.creator | Liu, S.H. and Shen, C.C. and Yi, Y.C. and Tsai, J.J. and Wang, C.C. and Chueh, J.T. and Lin, K.L. and Lee, T.C. and Pan, H.C. and Sheu, M.L. | - |
dc.creator | Liu, Shing Hwa;Shen, Chin Chang;Yi, Yu Chiao;Tsai, Jaw Ji;Wang, Chih Chien;Chueh, Ju Ting;Lin, Keh Liang;Lee, Tso Ching;Pan, Hung Chuan;Sheu, Meei Ling | en |
dc.date.accessioned | 2018-09-10T08:06:30Z | - |
dc.date.available | 2018-09-10T08:06:30Z | - |
dc.date.issued | 2010 | - |
dc.identifier.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-77954881040&partnerID=MN8TOARS | - |
dc.identifier.uri | http://scholars.lib.ntu.edu.tw/handle/123456789/355322 | - |
dc.description.abstract | Background and purpose: Peroxisome proliferator-activated receptor-γ (PPAR-γ), COX-2 and 15-lipoxygenase (LOX)-1 have been shown to be involved in tumour growth. However, the roles of PPAR-γ, COX-2 or 15-LOX-1 in gastric tumourigenesis remain unclear. Here, we investigate the role of 15-LOX-1 induction by honokiol, a small-molecular weight natural product, in PPAR-γ and COX-2 signalling during gastric tumourigenesis. Experimental approach: Human gastric cancer cell lines (AGS, MKN45, N87 and SCM-1) were cultured with or without honokiol. Gene and protein expressions were analysed by RT-PCR and Western blotting respectively. Small interfering RNAs (siRNAs) for COX-2, PPAR-γ and 15-LOX-1 were used to interfere with the expressions of these genes. A xenograft gastric tumour model in mouse was used for in vivo study. Key results: PPAR-γ and COX-2 proteins were highly expressed in gastric cancer cells. Inhibitors, or siRNA for COX-2 or PPAR-γ, significantly decreased cell viability. Honokiol markedly inhibited PPAR-γ and COX-2 expressions in gastric cancer cells and tumours of xenograft mice, and induced apoptosis and cell death. Honokiol markedly activated cellular 15-LOX-1 expression and 13-S-hydroxyoctadecadienoic acid (a primary product of 15-LOX-1 metabolism of linoleic acid) production. 15-LOX-1 siRNA could reverse the honokiol-induced down-regulation of PPAR-γ and COX-2, and cell apoptosis. 15-LOX-1 was markedly induced in tumours of xenograft mice treated with honokiol. Conclusions and implications: These findings suggest that induction of 15-LOX-1-mediated down-regulation of a PPAR-γ and COX-2 pathway by honokiol may be a promising therapeutic strategy for gastric cancer. ? 2010 The British Pharmacological Society. | - |
dc.language | en | en |
dc.relation.ispartof | British Journal of Pharmacology | en_US |
dc.source | AH | - |
dc.subject | 15-LOX-1; calpain; COX-2; gastric tumourigenesis; honokiol; PPAR-γ | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | arachidonate 15 lipoxygenase; arachidonate 15 lipoxygenase 1; coriolic acid; cyclooxygenase 2; honokiol; linoleic acid; peroxisome proliferator activated receptor gamma; small interfering RNA; unclassified drug; 13-hydroxy-9,11-octadecadienoic acid; ALOX15 protein, human; antineoplastic agent; arachidonate 15 lipoxygenase; biphenyl derivative; cyclooxygenase 2; honokiol; lignan; linoleic acid; peroxisome proliferator activated receptor gamma; prostaglandin synthase inhibitor; PTGS2 protein, human; animal experiment; animal model; apoptosis; article; cancer cell culture; cancer inhibition; cell death; cell viability; controlled study; down regulation; enzyme activation; enzyme induction; enzyme inhibition; gene expression; human; human tissue; immunohistochemistry; in vivo study; lipid metabolism; male; mouse; nonhuman; priority journal; protein expression; reverse transcription polymerase chain reaction; signal transduction; stomach cancer; Western blotting; xenograft; animal; Bagg albino mouse; cell survival; dose response; drug effects; drug screening; enzymology; gene expression regulation; genetics; metabolism; nude mouse; pathology; RNA interference; Stomach Neoplasms; time; tumor cell line; tumor volume; Animals; Antineoplastic Agents, Phytogenic; Apoptosis; Arachidonate 15-Lipoxygenase; Biphenyl Compounds; Blotting, Western; Cell Line, Tumor; Cell Survival; Cyclooxygenase 2; Cyclooxygenase Inhibitors; Dose-Response Relationship, Drug; Enzyme Activation; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Neoplastic; Humans; Lignans; Linoleic Acids; Male; Mice; Mice, Inbred BALB C; Mice, Nude; PPAR gamma; Reverse Transcriptase Polymerase Chain Reaction; RNA Interference; Signal Transduction; Stomach Neoplasms; Time Factors; Tumor Burden; Xenograft Model Antitumor Assays | - |
dc.title | Honokiol inhibits gastric tumourigenesis by activation of 15-lipoxygenase-1 and consequent inhibition of peroxisome proliferator-activated receptor-γ and COX-2-dependent signals | - |
dc.type | journal article | en |
dc.identifier.doi | 10.1111/j.1476-5381.2010.00804.x | - |
dc.relation.pages | 1963-1972 | - |
dc.relation.journalvolume | 160 | - |
dc.relation.journalissue | 8 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Toxicology | - |
crisitem.author.dept | Toxicology | - |
crisitem.author.orcid | 0000-0002-9976-1197 | - |
crisitem.author.orcid | 0000-0002-9976-1197 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 毒理學研究所 |
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