https://scholars.lib.ntu.edu.tw/handle/123456789/355323
Title: | Honokiol induces cell apoptosis in human chondrosarcoma cells through mitochondrial dysfunction and endoplasmic reticulum stress | Authors: | SHING-HWA LIU | Keywords: | Chondrosarcoma; ER; GRP78; Honokiol | Issue Date: | 2010 | Journal Volume: | 291 | Journal Issue: | 1 | Start page/Pages: | 20-30 | Source: | Cancer Letters | Abstract: | Chondrosarcoma is a malignant primary bone tumor that responds poorly to both chemotherapy and radiation therapy. In the present study, we investigated the anti-cancer effect of a honokiol, an active component isolated and purified from the Magnolia officinalis in human chondrosarcoma cells. Honokiol-induced cell apoptosis in human chondrosarcoma cell lines (including: JJ012 and SW1353) but not primary chondrocytes. Honokiol also induces upregulation of Bax and Bak, downregulation of Bcl-XL and dysfunction of mitochondria in chondrosarcoma cells. Honokiol triggered endoplasmic reticulum (ER) stress, as indicated by changes in cytosol-calcium levels. We also found that honokiol increased the expression and activities of glucose-regulated protein 78 (GRP78) and calpain. Transfection of cells with GRP78 or calpain siRNA reduced honokiol-mediated cell apoptosis in JJ012 cells. Importantly, animal studies have revealed a dramatic 53% reduction in tumor volume after 21 days of treatment. This study demonstrates that honokiol may be a novel anti-cancer agent targeting chondrosarcoma cells. ? 2009 Elsevier Ireland Ltd. All rights reserved. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-77949874077&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/355323 |
DOI: | 10.1016/j.canlet.2009.08.032 | SDG/Keyword: | calcium; calpain; glucose regulated protein 78; honokiol; protein Bak; protein Bax; protein bcl xl; small interfering RNA; animal experiment; animal model; antineoplastic activity; apoptosis; article; cancer size; cartilage cell; chondrosarcoma; controlled study; cytosol; disorders of mitochondrial functions; down regulation; drug effect; drug isolation; drug mechanism; drug targeting; endoplasmic reticulum stress; genetic transfection; human; human cell; magnolia officinalis; Magnoliaceae; nonhuman; priority journal; protein expression; sarcoma cell; upregulation; Animals; Antineoplastic Agents, Phytogenic; Apoptosis; Biphenyl Compounds; Bone Neoplasms; Calcium; Calpain; Cell Line, Tumor; Chondrosarcoma; Endoplasmic Reticulum; Heat-Shock Proteins; Humans; Lignans; Male; Mice; Mice, Inbred BALB C; Mitochondria; Xenograft Model Antitumor Assays; Animalia; Magnolia officinalis |
Appears in Collections: | 毒理學研究所 |
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