Immunogenicity of recombinant GP5 protein of porcine reproductive and respiratory syndrome virus expressed in tobacco plant
Resource
Veterinary Immunology and Immunopathology 135 (3-4): 234-242
Journal
Veterinary Immunology and Immunopathology
Journal Volume
135
Journal Issue
3-4
Pages
234-242
Date Issued
2010
Author(s)
Chia, M.-Y.
Hsiao, S.-H.
Chan, H.-T.
Huang, P.-L.
Tsai, Y.-C.
Lin, C.-M.
Abstract
The aim of the study was to evaluate the immunogenicity of the ORF5-encoded major envelop glycoprotein 5 (GP5) of porcine reproductive and respiratory syndrome virus (PRRSV) expressed in tobacco plant as a potential pig oral vaccine in protection against PRRSV infection. Six-week-old PRRSV-free pigs were fed four times orally with 50. g of chopped fresh GP5 transgenic tobacco leaves (GP5-T) (GP5 reaching 0.011% of total soluble protein) or wild-type tobacco leaves (W-T) each on days 0, 14, 28, and 42. Samples of serum, saliva, and peripheral blood mononuclear cells (PBMCs) were collected on days -1, 6, 13, 20, 27, 34, 41, and 48 after the initial oral vaccination. A similar vaccination-dependent gradual increase in the responses of serum and saliva anti-PRRSV total IgG and IgA, respectively, and in the levels of PRRSV-specific blastogenic response of PBMCs was seen in GP5-T-treated pigs; all statistically significant elevations occurred after the 2nd vaccination and were revealed after 20 days post-initial oral vaccination (DPIOV). Pigs fed on GP5-T also developed serum neutralizing antibodies to PRRSV at a titer of 1:4-1:8 after the 4th vaccination by 48 DPIOV. No detectable anti-PRRSV antibody responses and PRRSV-specific blastogenic response were seen in W-T-treated pigs. The present study has demonstrated that pigs fed on GP5-T could develop specific mucosal as well as systemic humoral and cellular immune responses against PRRSV. The results also support that transgenic plant as GP5-T can be an effective system for oral delivery of recombinant subunit vaccines in pigs. ? 2009 Elsevier B.V.
Subjects
Glycoprotein 5; PRRSV; Transgenic tobacco leaves
SDGs
Other Subjects
edible vaccine; immunoglobulin A antibody; immunoglobulin G antibody; neutralizing antibody; porcine reproductive and respiratory syndrome vaccine; recombinant glycoprotein gp 5; recombinant protein; unclassified drug; virus vaccine; animal experiment; antibody blood level; antibody response; antibody titer; article; cellular immunity; controlled study; humoral immunity; immunogenicity; lymphocyte proliferation; male; mucosal immunity; nonhuman; nucleotide sequence; open reading frame; peripheral blood mononuclear cell; porcine reproductive and respiratory syndrome; saliva analysis; tobacco; transgenic plant; virus neutralization; Administration, Oral; Animals; Antibodies, Neutralizing; Antibodies, Viral; Base Sequence; Bioreactors; DNA, Viral; Immunity, Cellular; Immunity, Humoral; Immunity, Mucosal; Immunoglobulin A, Secretory; Immunoglobulin G; Lymphocyte Activation; Male; Plants, Genetically Modified; Porcine Reproductive and Respiratory Syndrome; Porcine respiratory and reproductive syndrome virus; Saliva; Sus scrofa; Swine; Tobacco; Vaccines, Edible; Viral Envelope Proteins; Viral Vaccines; Nicotiana obtusifolia; Nicotiana tabacum; Porcine respiratory and reproductive syndrome virus; Suidae
Type
journal article
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