https://scholars.lib.ntu.edu.tw/handle/123456789/362209
DC Field | Value | Language |
---|---|---|
dc.contributor.author | HUI-WEN CHEN | en_US |
dc.contributor.author | Wang, C.-H. | en_US |
dc.contributor.author | IVAN-CHEN CHENG | en_US |
dc.creator | Chen, H.-W.;Wang, C.-H.;Cheng, I.-C. | - |
dc.date.accessioned | 2018-09-10T08:33:43Z | - |
dc.date.available | 2018-09-10T08:33:43Z | - |
dc.date.issued | 2011 | - |
dc.identifier.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-79952185399&partnerID=MN8TOARS | - |
dc.identifier.uri | http://scholars.lib.ntu.edu.tw/handle/123456789/362209 | - |
dc.description.abstract | Infectious bronchitis virus (IBV) infection has been a major threat to the poultry industry worldwide. Current commercially available ELISA kits detect group-specific antibodies; however, to understand the status of field infection, a monoclonal antibody (mAb) blocking ELISA (b-ELISA) against local IBVs was developed. The selected mAb showed specificity to Taiwan IBV strains but had no cross-reactivity with the vaccine strain H120. Using the hemagglutination inhibition (HI) test as a standard, the cut-off value, sensitivity, and specificity of a b-ELISA using this mAb were evaluated in 390 field samples. The type-specificity of detection was validated using a panel of chicken hyperimmune sera. The results showed that the b-ELISA demonstrated high sensitivity (98.0%) and specificity (97.2%) of detection. The agreement between the results of the b-ELISA and the HI test was statistically significant (Kappa. =0.95), and there was no significant difference between these two methods (McNemar p=0.72). The b-ELISA specifically detected Taiwan IBV serotypes but not three non-Taiwan IBV serotypes nor sera against other avian pathogens. This b-ELISA provides type-specific antibody detection of local IBV strains. It has the potential to serve as a rapid and reliable diagnostic method for IBV clinical infections in the field in Taiwan. ? 2011 Elsevier B.V. | - |
dc.language | en | en |
dc.relation.ispartof | Journal of Virological Methods | - |
dc.source | AH | - |
dc.subject | Blocking ELISA; Hemagglutination inhibition test; Infectious bronchitis virus; Monoclonal antibody | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | monoclonal antibody; monoclonal antibody QI3 4; unclassified drug; animal cell; antibody detection; antibody specificity; article; Avian infectious bronchitis virus; blocking enzyme linked immunosorbent assay; controlled study; enzyme linked immunosorbent assay; hemagglutination inhibition test; intermethod comparison; nonhuman; priority journal; reference value; sensitivity and specificity; serotype; Taiwan; virus strain; Animals; Antibodies, Monoclonal; Antibodies, Viral; Chickens; Coronavirus Infections; Enzyme-Linked Immunosorbent Assay; Infectious bronchitis virus; Poultry Diseases; Sensitivity and Specificity; Taiwan; Virology; Aves; Avian infectious bronchitis virus | - |
dc.title | A type-specific blocking ELISA for the detection of infectious bronchitis virus antibody | - |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.jviromet.2010.12.016 | - |
dc.relation.pages | 7-12 | - |
dc.relation.journalvolume | 173 | - |
dc.relation.journalissue | 1 | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
crisitem.author.dept | Veterinary Medicine | - |
crisitem.author.orcid | 0000-0002-0595-3420 | - |
crisitem.author.orcid | 0000-0002-0168-3902 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
Appears in Collections: | 獸醫學系 |
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