https://scholars.lib.ntu.edu.tw/handle/123456789/362284
Title: | Immunopathogenic behaviors of canine transmissible venereal tumor in dogs following an immunotherapy using dendritic/tumor cell hybrid | Authors: | Pai, C.-C. TZONG-FU KUO Mao, S.J.T. Chuang, T.-F. CHEN-SI LIN Chu, R.-M. |
Keywords: | Bone-marrow derived dendritic cells (BMDCs); Canine transmissible venereal tumor (CTVT); Tumor vaccine | Issue Date: | 2011 | Journal Volume: | 139 | Journal Issue: | 2-4 | Start page/Pages: | 187-199 | Source: | Veterinary Immunology and Immunopathology | Abstract: | Canine transmissible venereal tumor (CTVT) is a naturally occurring tumor that can be transmitted between dogs via live tumor cell inoculation. It is also a spontaneous self-regression tumor and its behavior is closely related to host immune responses. Since CTVT had been widely used for tumor models in canine cancers, whether this self-regression may overtake the immunity elicited from an exogenous tumor vaccine remains unclear and certainly worthwhile to be investigated. In this study, we used DCs/tumor hybrids as a tumor vaccine to evaluate the CTVT model. We prepared mature allogeneic dendritic cells from bone marrow and then assessed their phenotype (CD80, CD83, CD86, CD1a, CD11c, CD40 and MHC II), antigen uptake and presenting abilities. Fused dendritic cell/CTVT hybrids were then used as a vaccine, administered three times at two-week intervals via subcutaneous injection near the bilateral auxiliary and inguinal lymph nodes. In comparison with unvaccinated dogs (spontaneous regressed group), within a period of 2.5 months, the vaccinations substantially inhibited tumor progression (p< 0.05) and accelerated the rate of regression by a mechanism involving amplification of the host tumor-specific adaptive immune responses and NK cytotoxicity (p< 0.001). Pathologic examination revealed early massive lymphocyte infiltration resulting in final tumor necrosis. In addition, there are not any detectable effects on routine physical, body temperature or blood chemistry examinations. In conclusion, our data furnishes a reference value showing that CTVT is a model of potential use for the study of immunity elicited by vaccines against tumors, and also enable early-phase evaluation of the dendritic cell/tumor vaccine in terms of raising host immunity. ? 2010 Elsevier B.V. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-78650908661&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/362284 |
DOI: | 10.1016/j.vetimm.2010.10.013 | SDG/Keyword: | B7 antigen; CD40 antigen; CD83 antigen; CD86 antigen; dendritic cell vaccine; glycoprotein p 15095; major histocompatibility antigen class 2; t6 antigen; tumor cell vaccine; allogenic bone marrow transplantation; animal experiment; animal model; animal venereal tumor; article; blood chemistry; body temperature; cancer immunotherapy; canine transmissible venereal tumor; comparative study; control group; controlled study; cytotoxicity; dog; experimental model; female; immune response; immunopathogenesis; inguinal lymph node; lymphocytic infiltration; male; mixed lymphocyte reaction; natural killer cell; nonhuman; phenotype; tumor growth; vaccination; Animals; Cancer Vaccines; Dendritic Cells; Dog Diseases; Dogs; Female; Hybrid Cells; Macrophages; Male; Venereal Tumors, Veterinary; Canis familiaris |
Appears in Collections: | 獸醫學系 |
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