Detection of porcine circovirus type 1 in commercial porcine vaccines by loop-mediated isothermal amplification
Journal
Folia Microbiologica
Journal Volume
56
Journal Issue
6
Pages
483-489
Date Issued
2011
Author(s)
Abstract
A loop-mediated isothermal amplification (LAMP) method with a real-time monitoring system was developed for the detection of porcine circovirus type 1 (PCV1) in commercial swine vaccines. This method was highly specific for PCV1. No cross-reaction to porcine circovirus type 2, porcine parvovirus, pseudorabies virus, classical swine fever virus, and porcine reproductive and respiratory syndrome virus was observed. The analytical sensitivity of the LAMP for PCV1 DNA was 10 copies/μl in the case of positive recombinant plasmid comparable to that obtained from the nested polymerase chain reaction (nested PCR). Furthermore, 25 commercial swine vaccines were tested by both the LAMP and the nested PCR, and three of them were tested positive for PCV1 DNA. These results indicate that PCV1 DNA can be real-time detected by the LAMP; the method was highly specific, sensitive, and rapid for the detection of PCV1 DNA, particularly in commercial swine vaccines. ? 2011 The Author(s).
SDGs
Other Subjects
primer DNA; virus vaccine; animal; animal disease; article; Circoviridae infection; Circovirus; classification; evaluation; genetics; isolation and purification; methodology; nucleic acid amplification; swine; swine disease; virology; Animals; Circoviridae Infections; Circovirus; DNA Primers; Nucleic Acid Amplification Techniques; Swine; Swine Diseases; Viral Vaccines; Arterivirus; Circovirus; Classical swine fever virus; Pestivirus; Porcine circovirus 1; Porcine circovirus 2; Porcine parvovirus; Porcine respiratory and reproductive syndrome virus; Suid herpesvirus 1; Suidae; Sus
Type
journal article