https://scholars.lib.ntu.edu.tw/handle/123456789/362771
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Chia, M.-Y. | en_US |
dc.contributor.author | Hsiao, S.-H. | en_US |
dc.contributor.author | Chan, H.-T. | en_US |
dc.contributor.author | YI-YIN DO | en_US |
dc.contributor.author | Huang, P.-L. | en_US |
dc.contributor.author | HUI-WEN CHANG | en_US |
dc.contributor.author | Tsai, Y.-C. | en_US |
dc.contributor.author | Lin, C.-M. | en_US |
dc.contributor.author | VICTOR FEI PANG | en_US |
dc.contributor.author | CHIAN-REN JENG | en_US |
dc.creator | Chia, M.-Y.;Hsiao, S.-H.;Chan, H.-T.;Do, Y.-Y.;Huang, P.-L.;Chang, H.-W.;Tsai, Y.-C.;Lin, C.-M.;Pang, V.F.;Jeng, C.-R. | - |
dc.date.accessioned | 2018-09-10T08:35:30Z | - |
dc.date.available | 2018-09-10T08:35:30Z | - |
dc.date.issued | 2011 | - |
dc.identifier.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-79952183287&partnerID=MN8TOARS | - |
dc.identifier.uri | http://scholars.lib.ntu.edu.tw/handle/123456789/362771 | - |
dc.description.abstract | Escherichia coli heat-labile enterotoxin B subunit (LTB) can be used as an adjuvant for co-administered antigens. Our previous study showed that the expression of neutralizing epitope GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) in transgenic tobacco plant (GP5-T) could induce PRRSV-specific immune responses in pigs. A transgenic tobacco plant co-expressing LTB and PRRSV GP5 as a fusion protein (LTB-GP5-T) was further constructed and its immunogenicity was evaluated. Pigs were given orally three consecutive doses of equal concentration of recombinant GP5 protein expressed in leaves of LTB-GP5-T or GP5-T at a 2-week interval and challenged with PRRSV at 7 weeks post-initial immunization. Pigs receiving LTB-GP5-T or GP5-T developed PRRSV-specific antibody- and cell-mediated immunity and showed significantly lower viremia and tissue viral load and milder lung lesions than wild type tobacco plant (W-T). The LTB-GP5-T-treated group had relatively higher immune responses than the GP5-T-treated group, although the differences were not statistically significant. ? 2011 Elsevier B.V. | - |
dc.language | en | en |
dc.relation.ispartof | Veterinary Immunology and Immunopathology | - |
dc.source | AH | - |
dc.subject | GP5; LTB; PRRSV; Transgenic tobacco leaves | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | antibody; epitope; Escherichia coli enterotoxin; glycoprotein; protein subunit; recombinant protein; animal cell; animal experiment; animal tissue; antibody production; antibody specificity; article; cellular immunity; concentration (parameters); Escherichia coli; immune response; immunogenicity; lung lesion; male; nonhuman; nucleotide sequence; plant leaf; procine reproductive and respiratory syndrome virus; protein expression; respiratory virus; swine; tobacco; viremia; virus load; Adjuvants, Immunologic; Administration, Oral; Animals; Antibodies, Viral; Bacterial Toxins; Base Sequence; DNA Primers; Enterotoxins; Escherichia coli; Escherichia coli Proteins; Lymphocyte Activation; Male; Plant Leaves; Plants, Genetically Modified; Porcine Reproductive and Respiratory Syndrome; Porcine respiratory and reproductive syndrome virus; Recombinant Fusion Proteins; Saliva; Sus scrofa; Swine; Tobacco; Vaccines, Edible; Vaccines, Synthetic; Viral Envelope Proteins; Viral Load; Viral Vaccines; Escherichia coli; Nicotiana obtusifolia; Nicotiana tabacum; Porcine respiratory and reproductive syndrome virus; Suidae | - |
dc.title | Evaluation of the immunogenicity of a transgenic tobacco plant expressing the recombinant fusion protein of GP5 of porcine reproductive and respiratory syndrome virus and B subunit of Escherichia coli heat-labile enterotoxin in pigs | - |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.vetimm.2011.01.002 | - |
dc.relation.pages | 215-225 | - |
dc.relation.journalvolume | 140 | - |
dc.relation.journalissue | 3-4 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Horticulture and Landscape Architecture | - |
crisitem.author.dept | Master Program in Global Agriculture Technology and Genomic Science (Global ATGS) | - |
crisitem.author.dept | Molecular and Comparative Pathobiology | - |
crisitem.author.dept | Veterinary Medicine | - |
crisitem.author.dept | Molecular and Comparative Pathobiology | - |
crisitem.author.orcid | 0000-0002-2142-2486 | - |
crisitem.author.orcid | 0000-0001-8877-1886 | - |
crisitem.author.orcid | 0000-0002-8048-4548 | - |
crisitem.author.orcid | 0000-0003-3941-6276 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | International College | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
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