https://scholars.lib.ntu.edu.tw/handle/123456789/364186
標題: | The down-regulation of galectin-1 expression is a specific biomarker of arsenic toxicity | 作者: | Chang, Y.-Y. Chiang, M.-C. Kuo, T.-C. Chi, L.-L. Kao, Y.-H. Huang, R.-N. RONG-NAN HUANG |
關鍵字: | Antimony; Arsenic; Cadmium; Galectin-1; Nickel | 公開日期: | 2011 | 卷: | 205 | 期: | 1 | 起(迄)頁: | 38-46 | 來源出版物: | Toxicology Letters | 摘要: | Galectin-1 (GAL1) is known as a β-galactoside-binding protein that also can bind with arsenic to regulate cell functions. Using RNA interference technique, we investigated the possible mechanism involved in GAL1 modulation of arsenite-inhibited cell survival in 3T3 fibroblast and KB oral cancer cells. GAL1 gene knockdown significantly attenuated sodium arsenite (NaAsO2) and arsenic trioxide (As2O3) inhibition of cell survival. However, GAL1 gene knockdown did not alter the inhibition of cell survival by antimony chloride, cadmium chloride or nickel sulfate. These results suggested the GAL1 selectively affects particular types of heavy metal elements. Flow cytometric analysis indicated GAL1 gene knockdown also suppressed As(III)-stimulated levels of sub-G1 and G2/M growth arrest in both cells. Moreover, atomic absorption spectrophotometric results showed that GAL1 gene knockdown reduced the total arsenic accumulation of both cells after the NaAsO2 and As2O3 treatment. These results suggested that GAL1 gene knockdown mediates the apoptotic effects of arsenic in 3T3 and KB cells via regulation of the cellular arsenic levels. We propose that down-regulation of GAL1 expression may be a useful and specific biomarker in assessing the toxicity of arsenic exposure. ? 2011 Elsevier Ireland Ltd. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-79959998200&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/364186 |
DOI: | 10.1016/j.toxlet.2011.05.002 | SDG/關鍵字: | antimony; arsenic trioxide; arsenite sodium; cadmium chloride; galectin 1; nickel sulfate; apoptosis; arsenic poisoning; article; atomic absorption spectrometry; cancer cell; cell cycle arrest; cell cycle G1 phase; cell cycle G2 phase; cell cycle M phase; cell strain 3T3; cell survival; concentration (parameters); controlled study; down regulation; flow cytometry; gene silencing; human; human cell; molecular mechanics; priority journal; protein expression; regulatory mechanism; 3T3 Cells; Animals; Antimony; Arsenic; Arsenic Poisoning; Biological Markers; Blotting, Western; Cadmium; Cell Cycle; Down-Regulation; Flow Cytometry; Galectin 1; Humans; KB Cells; Mice; Nickel; RNA Interference; Spectrophotometry, Atomic; Tetrazolium Salts; Thiazoles; Transfection |
顯示於: | 昆蟲學系 |
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