https://scholars.lib.ntu.edu.tw/handle/123456789/365239
標題: | Differentiation of foot-and-mouth disease-infected pigs from vaccinated pigs using antibody-detecting sandwich ELISA | 作者: | Chen, T.-H. Lee, F. Lin, Y.-L. Dekker, A. Chung, W.-B. Pan, C.-H. Jong, M.-H. Huang, C.-C. Lee, M.-C. HSIANG-JUNG TSAI |
關鍵字: | Antibody detection; Foot-and-mouth disease; Non-structural protein; Sandwich ELISA | 公開日期: | 2011 | 卷: | 73 | 期: | 8 | 起(迄)頁: | 977-984 | 來源出版物: | Journal of Veterinary Medical Science | 摘要: | The presence of serum antibodies for nonstructural proteins of the foot-and-mouth disease virus (FMDV) can differentiate FMDV-infected animals from vaccinated animals. In this study, a sandwich ELISA was developed for rapid detection of the foot-andmouth disease (FMD) antibodies; it was based on an Escherichia coli-expressed, highly conserved region of the 3ABC nonstructural protein of the FMDV O/TW/99 strain and a monoclonal antibody derived from the expressed protein. The diagnostic sensitivity of the assay was 98.4%, and the diagnostic specificity was 100% for na?ve and vaccinated pigs; the detection ability of the assay was comparable those of the PrioCHECK and UBI kits. There was 97.5, 93.4 and 66.6% agreement between the results obtained from our ELISA and those obtained from the PrioCHECK, UBI and CHEKIT kits, respectively. The kappa statistics were 0.95, 0.87 and 0.37, respectively. Moreover, antibodies for nonstructural proteins of the serotypes A, C, Asia 1, SAT 1, SAT 2 and SAT 3 were also detected in bovine sera. Furthermore, the absence of cross-reactions generated by different antibody titers against the swine vesicular disease virus and vesicular stomatitis virus (VSV) was also highlighted in this assay's specificity. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-80052603901&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/365239 |
DOI: | 10.1292/jvms.10-0351 | SDG/關鍵字: | 3ABC protein, virus; virus antibody; virus protein; animal; animal disease; article; blood; cross reaction; enzyme linked immunosorbent assay; foot and mouth disease; Foot and mouth disease virus; immunology; methodology; sensitivity and specificity; swine; swine disease; vaccination; Animals; Antibodies, Viral; Cross Reactions; Enzyme-Linked Immunosorbent Assay; Foot-and-Mouth Disease; Foot-and-Mouth Disease Virus; Sensitivity and Specificity; Swine; Swine Diseases; Vaccination; Viral Nonstructural Proteins; Animalia; Bovinae; Escherichia coli; Foot-and-mouth disease virus; Suidae; Swine vesicular disease virus; Vesicular stomatitis virus |
顯示於: | 獸醫學系 |
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