https://scholars.lib.ntu.edu.tw/handle/123456789/365966
標題: | Protein A-conjugated luminescent gold nanodots as a label-free assay for immunoglobulin G in plasma | 作者: | Shiang, Y.-C. Lin, C.-A. Huang, C.-C. Chang, H.-T. HUAN-TSUNG CHANG |
公開日期: | 2011 | 卷: | 136 | 期: | 6 | 起(迄)頁: | 1177-1182 | 來源出版物: | Analyst | 摘要: | We have employed protein A-modified gold nanodots (PA-Au NDs) as a luminescence sensor for the detection of human immunoglobulin G (hIgG) in homogeneous solutions. The luminescent PA-Au NDs were prepared simply by mixing protein A with the luminescent Au NDs (average diameter: ca. 1.8 nm). The specific interactions that occur between protein A and hIgG allowed us to use the PA-Au NDs to detect hIgG selectively. Under optimal conditions [10 nM PA-Au NDs (two protein A molecules per Au ND), 5.0 mM phosphate buffer solution, pH 7.4], the PA-Au ND probe detected hIgG with high sensitivity (limit of detection = 10 nM) and remarkable selectivity (>50-fold) over other proteins. In an assay that took advantage of the competition between protein G and the PA-Au NDs for IgG, we detected protein G at concentrations as low as 85 nM. This PA-Au ND probe allowed determination of the hIgG concentration in plasma samples without any need for sample pretreatment. Our results exhibited a good linear correlation (R2 = 0.97) with those obtained using an enzyme-linked immunosorbent assay. Our simple, sensitive, and selective approach appears to hold practical potential for use in the clinical diagnosis of immune diseases associated with changes in hIgG levels. ? 2011 The Royal Society of Chemistry. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-79952076427&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/365966 |
DOI: | 10.1039/c0an00889c |
顯示於: | 化學系 |
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