Induction of apoptosis in human breast adenocarcinoma cells MCF-7 by monapurpyridine a, a new azaphilone derivative from monascus purpureus NTU 568
Resource
MOLECULES, 17(1), 664-673
Journal
Molecules
Journal Volume
17
Journal Issue
1
Pages
664-673
Date Issued
2012
Author(s)
Hu, Kuang-Chun
Hsu, LC
Hsu, YW
Wang, Horng-Yuan
Liang, YH
Liu, Sung-Chen
Liaw, CC
Liu, Chuan-Chuan
Hung, Chung-Lieh
Kuo, YH
Bair, Ming-Jong
Pan, TM.
Liu, Chun-Jen
Wu, Ming-Shiang
Shih, Shou-Chuan
Abstract
A new azaphilonidal derivative, monapurpyridine A (MPA), has recently been isolated from the fermented products of Monascus purpureus NTU 568. The structure of MPA was elucidated by nuclear magnetic resonance ( 1H-NMR, 13C-NMR, COSY, HMQC, and HMBC) and other spectroscopic analyses. Biological evaluation revealed that MPA could induce cell death in human breast adenocarcinoma cells MCF-7, and it has no significant toxicity to normal mammary epithelial cells M10. The MTT assay and flow cytometric analysis were employed to investigate cell viability and cell cycle influenced by MPA. Moreover, we used Western blot and caspase activity assay to demonstrate the activation of caspase-3, -8 and -9 resulted from MPA. All evidence supported that MPA was suitable for developing into a chemotherapeutic or chemopreventive agent against breast cancer. ? 2012 by the authors.
Subjects
Apoptosis; Azaphilone; Cytotoxicity; Monapurpyridine A; Monascus purpureus NTU 568
SDGs
Other Subjects
antineoplastic agent; caspase; monapurpyridine A; pyridine derivative; adenocarcinoma; apoptosis; article; breast tumor; cell survival; chemical structure; chemistry; culture medium; drug effect; enzyme activation; female; fermentation; human; isolation and purification; metabolism; Monascus; rice; tumor cell line; Adenocarcinoma; Antineoplastic Agents; Apoptosis; Breast Neoplasms; Caspases; Cell Line, Tumor; Cell Survival; Culture Media; Enzyme Activation; Female; Fermentation; Humans; Molecular Structure; Monascus; Oryza sativa; Pyridines
Type
journal article