https://scholars.lib.ntu.edu.tw/handle/123456789/369496
標題: | Lactobacillus kefiranofaciens M1 isolated from milk kefir grains ameliorates experimental colitis in vitro and in vivo | 作者: | Chen, Y.P. Hsiao, P.J. Hong, W.S. Dai, T.Y. Chen, M.J. MING-JU CHEN |
關鍵字: | Experimental colitis; Intestinal epithelial cell; Kefir; Probiotic | 公開日期: | 2012 | 卷: | 95 | 期: | 1 | 起(迄)頁: | 63-74 | 來源出版物: | Journal of Dairy Science | 摘要: | Lactobacillus kefiranofaciens M1, isolated from and identified in Taiwanese milk kefir grain, has demonstrated immune-modulating activity. In the present study, we further investigated the effects of Lb. kefiranofaciens M1 on intestinal epithelial cells in vitro and on dextran sodium sulfate (DSS)-induced colitis in vivo. The possible mechanisms regarding the cytokine products and intestinal epithelial barrier restoration as well as the putative receptor for the protective effects of Lb. kefiranofaciens M1 were investigated. In vitro results indicated that Lb. kefiranofaciens M1 could strengthen the epithelial barrier function in vitro by increasing the transepithelial electrical resistance (TEER) and significantly upregulated the level of the chemokine CCL-20 at both the apical and basolateral sites. The in vivo effects of Lb. kefiranofaciens M1 on the regulation of intestinal physiology indicate that this strain could ameliorate DSS-induced colitis with a significant attenuation of the bleeding score and colon length shortening. Production of proinflammatory cytokines was decreased and that of the antiinflammatory cytokine IL-10 was increased in the DSS-treated mice given Lb. kefiranofaciens M1. The putative receptor for the protective effects of Lb. kefiranofaciens M1 was toll-like receptor 2 (TLR2), which was involved in probiotic-induced cytokine production in vitro and in attenuation of the bleeding score and colon length shortening in vivo. In this study, the kefir lactobacillus Lb. kefiranofaciens M1 clearly demonstrated an anticolitis effect. Based on these results, Lb. kefiranofaciens M1 has the potential to be applied in fermented dairy products as an alternative therapy for intestinal disorders. ? 2012 American Dairy Science Association. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84055192823&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/369496 |
DOI: | 10.3168/jds.2011-4696 | SDG/關鍵字: | cytokine; animal; article; C57BL mouse; cell strain CACO 2; cell strain HEK293; colitis; dairy product; disease model; drug effect; female; human; intestine; intestine mucosa; isolation and purification; Lactobacillus; metabolism; microbiology; mouse; organ culture technique; pathology; reporter gene; Animals; Caco-2 Cells; Colitis; Cultured Milk Products; Cytokines; Disease Models, Animal; Female; Genes, Reporter; HEK293 Cells; Humans; Intestinal Mucosa; Intestines; Lactobacillus; Mice; Mice, Inbred C57BL; Organ Culture Techniques |
顯示於: | 動物科學技術學系 |
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