Comparison of intradermal tests, total serum IgE concentrations and allergen-specific IgE using an arrayed protein chip in atopic dogs
Journal
Journal of Animal and Veterinary Advances
Journal Volume
11
Journal Issue
18
Pages
3298-3303
Date Issued
2012
Author(s)
Abstract
Canine atopic dermatitis is a common pruritic skin disease which is most commonly associated with Immunoglobulin (Ig) E immune response to environmental allergens. The aims of this study were to report comparison between total serum IgE concentrations in atopic and non-atopic dogs. Comparison between results of intradermal tests and serum allergen-specific IgE arrayed protein chips was also investigated. Total serum IgE concentrations between 37 atopic dogs (92.5±102.3 μg mL-1) and 34 non-atopic dogs (16.2±30.2 μg mL-1) were significantly different (p<0.001). A positive correlation between total serum IgE concentrations and corresponding modified canine atopic dermatitis extent and severity index scores was found (r = 0.56, p<0.001) in atopic dogs. The cut-off value was 16.8 jag mL-1 with the sensitivity of 91.9% and the specificity of 85.3%. The overall sensitivity of specific IgE serum tests was 24%, specificity was 95% and efficacy was 81%. Total serum IgE concentrations could be a screening test and determine severity of atopic dermatitis in dogs with discrete clinical evaluation. However, arrayed protein chip method might not be suitable for screening functional allergen-specific IgE. © Medwell Journals, 2012.
Type
journal article