https://scholars.lib.ntu.edu.tw/handle/123456789/377079
標題: | Taurine homeostasis requires de novo synthesis via cysteine sulfinic acid decarboxylase during zebrafish early embryogenesis | 作者: | Chang, Y.-C. Ding, S.-T. Lee, Y.-H. Wang, Y.-C. Huang, M.-F. Liu, I.-H. I-HSUAN LIU SHIH-TORNG DING |
關鍵字: | Csad; Cysteine sulfinic acid decarboxylase; Embryogenesis; Taurine; Zebrafish | 公開日期: | 2013 | 卷: | 44 | 期: | 2 | 起(迄)頁: | 615-629 | 來源出版物: | Amino Acids | 摘要: | Cysteine sulfinic acid decarboxylase (Csad) is the rate-limiting enzyme in the de novo biosynthesis of taurine. There are a number of physiological roles of taurine, such as bile salt synthesis, osmoregulation, lipid metabolism, and oxidative stress inhibition. To investigate the role of de novo synthesis of taurine during embryonic development, zebrafish csad was cloned and functionally analyzed. Semi-quantitative RT-PCR showed that csad transcripts are maternally deposited, while whole-mount in situ hybridization demonstrated that csad is expressed in yolk syncytial layer and various embryonic tissues such as notochord, brain, retina, pronephric duct, liver, and pancreas. Knockdown of csad significantly reduced the embryonic taurine level, and the affected embryos had increased early mortality and cardiac anomalies. mRNA coinjection and taurine supplementation rescued the cardiac phenotypes suggesting that taurine originating from the de novo synthesis pathway plays a role in cardiac development. Our findings indicated that the de novo synthesis pathway via Csad plays a critical role in taurine homeostasis and cardiac development in zebrafish early embryos. ? 2012 Springer-Verlag. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84878353594&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/377079 |
DOI: | 10.1007/s00726-012-1386-8 | SDG/關鍵字: | carboxylyase; cysteine sulfinate decarboxylase; messenger RNA; taurine; unclassified drug; amino acid analysis; amino acid synthesis; animal tissue; article; controlled study; embryo; embryo development; enzyme activity; female; homeostasis; human; human cell; in situ hybridization; male; molecular cloning; mortality; nonhuman; nucleotide sequence; priority journal; protein expression; quantitative analysis; reverse transcription polymerase chain reaction; RNA sequence; sequence analysis; signal transduction; tissue level; zebra fish; Animals; Carboxy-Lyases; Embryonic Development; Female; Fish Proteins; Homeostasis; Male; Taurine; Zebrafish; Danio rerio |
顯示於: | 動物科學技術學系 |
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