https://scholars.lib.ntu.edu.tw/handle/123456789/377234
標題: | Prussian blue nanoparticles as nanocargoes for delivering DNA drugs to cancer cells | 作者: | LIN-CHI CHEN | 關鍵字: | 11-mercaptoundecanoic acid (MUA); decoy oligodeoxynucleotide (dODN); drug delivery system (DDS); prostate cancer cell; Prussian blue nanoparticles (PBNPs) | 公開日期: | 2013 | 卷: | 14 | 期: | 4 | 來源出版物: | Science and Technology of Advanced Materials | 摘要: | We studied the use of Prussian blue nanoparticles (PBNPs) as novel nanocarriers for sending DNA drugs into cancer cells. 11-mercaptoundecanoic acid (MUA) was used to functionalize the surfaces of PBNPs (nanocubes with an average dimension of 75 nm) for subsequent covalent grafting of a 33-mer DNA drug with a FAM reporter at the 3′ end. The PBNPs synthesis and DNA drug conjugation were characterized by transmission electron microscopy (TEM) and Fourier-transform infrared absorption (FTIR), respectively. The drug was a decoy oligodeoxynucleotide (dODN) that inhibits the signal transducer and activator of transcription 3 (STAT3). The DNA-PBNPs drug (dODN@MUA-PBNPs) was delivered into human prostate carcinoma 22rv1 cells by endocytosis in vitro as confirmed by confocal fluorescence microscopy. MTT cell viability assays were carried out to assess the effect of the DNA-PBNPs drug. The results showed that the dODN molecules were successfully conjugated to the MUA modified PBNPs via amide and/or disulfide bond formation and could thus be successfully delivered into the cancer cells. The control experiments showed that the unconjugated dODN molecules were not able to enter the cancer cells no matter whether non-functionalized PBNPs were present or not. It was also found that the DNA-PBNPs drugs were internalized and then distributed homogeneously throughout the cell, including cytoplasmic and nucleic regions, after endocytosis. The cancer cell-killing ability increased with the amount of dODN conjugated on PBNPs and the dosage of DNA-PBNPs drug internalized. ? 2013 National Institute for Materials Science. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84884860355&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/377234 |
DOI: | 10.1088/1468-6996/14/4/044405 | SDG/關鍵字: | 11-mercaptoundecanoic acid; Drug delivery system; Oligodeoxynucleotides; Prostate cancer cells; Prussian blue nanoparticles; Cells; Covalent bonds; DNA; Drug delivery; Fluorescence microscopy; Fourier transform infrared spectroscopy; Light absorption; Molecular biology; Molecules; Nanoparticles; Transmission electron microscopy; Drug dosage |
顯示於: | 生物機電工程學系 |
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