https://scholars.lib.ntu.edu.tw/handle/123456789/377588
Title: | Upregulated hPuf-A promotes breast cancer tumorigenesis | Authors: | Chi-Chen Fan Liek-Yeow Lee Ming-Ying Yu Chi-Yuan Tzen Chi Chou MAU-SUN CHANG |
Keywords: | Breast cancer; DDX3; hPuf-A; Puf; RbAp48 | Issue Date: | 2013 | Journal Volume: | 34 | Journal Issue: | 5 | Start page/Pages: | 2557 - 2564 | Source: | Tumor Biology | Abstract: | hPuf-A is a member of RNA-binding PUF family that regulates mRNA translation. Redistribution of hPuf-A from the nucleolus to the nucleoplasm upon genotoxic stress modulates the poly(ADP-ribosyl)ation activity of PARP-1. Here, we report a novel function of hPuf-A involved in promoting breast cancer progression. Immunohistochemical studies showed higher expression levels of hPuf-A in stage I, II, III, and IV breast cancer specimens in contrast with those of hPuf-A in ductal carcinoma in situ. The presence of hPuf-A is highly associated with colony formation capacities in breast cancer T47D and MDA-MB-231 cells. Xenograft growth of hPuf-A-silenced and hPuf-A overexpressing MDA-MB-231 cells in nude mice was substantially in concert with colony formation capacities. This promoting effect of hPuf-A in tumorigenesis might be correlated with the regulation of its associated mRNAs, such as RbAp48 and DDX3. Collectively, hPuf-A may have diagnostic values in breast cancer progression. ? 2013 International Society of Oncology and BioMarkers (ISOBM). |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84885423249&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/377588 |
ISSN: | 1423-0380 | DOI: | 10.1007/s13277-013-0801-4 |
Appears in Collections: | 生化科學研究所 |
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