https://scholars.lib.ntu.edu.tw/handle/123456789/378598
Title: | Inhibitory potential of Grifola frondosa bioactive fractions on α-amylase and α-glucosidase for management of hyperglycemia | Authors: | Su, C.-H. Lu, T.-M. Lai, M.-N. Ng, L.-T. LEAN-TEIK HUANG |
Keywords: | α-amylase; α-glucosidase; bioactive components; Grifola frondosa; medicinal mushroom | Issue Date: | 2013 | Journal Volume: | 60 | Journal Issue: | 4 | Start page/Pages: | 446-452 | Source: | Biotechnology and Applied Biochemistry | Abstract: | This study examined the inhibitory effects of Grifola frondosa (GF), a medicinal mushroom popularly consumed in traditional medicine and health food, on digestive enzymes related to type 2 diabetes; chemical profiles and inhibitory kinetics of its bioactive fractions were also analyzed. Results showed that all GF extracts showed weak anti-α-amylase activity; however, strong anti-α-glucosidase activity was noted on GF n-hexane extract (GF-H). Further fractionation confirmed that compared with acarbose (a commercial α-glucosidase inhibitor), the nonpolar fraction of GF possessed a stronger anti-α-glucosidase activity but a weaker anti-α-amylase activity. These activities were not derived from ergosterol and ergosterol peroxide, two major compounds of this fraction. The inhibitory kinetics of GF-H on α-glucosidase was competitive inhibition. GF-H was as good as acarbose in inhibiting the starch digestion in vitro. Oleic acid and linoleic acid could be the major active constituents that have contributed to the potency of GF in inhibiting α-glucosidase activity. ? 2013 International Union of Biochemistry and Molecular Biology, Inc. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84883165061&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/378598 |
DOI: | 10.1002/bab.1105 | SDG/Keyword: | Active constituents; Bioactive components; Competitive inhibition; Glucosidase; Glucosidase activity; Glucosidase inhibitors; Grifola frondosa; Medicinal mushroom; Chemical analysis; Linoleic acid; Plants (botany); Amylases; acarbose; alpha glucosidase; amylase; ergosterol; Grifola frondosa extract; hexane; linoleic acid; oleic acid; plant extract; starch; unclassified drug; article; competitive inhibition; controlled study; drug mechanism; enzyme activity; fractionation; Grifola frondosa; hyperglycemia; medicinal mushroom; non insulin dependent diabetes mellitus; nonhuman; bioactive components; Grifola frondosa; medicinal mushroom; α-amylase; α-glucosidase; alpha-Amylases; alpha-Glucosidases; Digestion; Enzyme Inhibitors; Grifola; Hydrolysis; Hyperglycemia; Kinetics; Starch |
Appears in Collections: | 農業化學系 |
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