https://scholars.lib.ntu.edu.tw/handle/123456789/380243| Title: | Development of a Luminex assay for the detection of swine antibodies to non-structural proteins of foot-and-mouth disease virus | Authors: | Chen, T.-H. Lee, F. Lin, Y.-L. Pan, C.-H. Shih, C.-N. Lee, M.-C. Tsai, H.-J. HSIANG-JUNG TSAI |
Keywords: | Antibody detection; Enzyme-linked immunosorbent assay; Foot-and-mouth disease virus; Luminex assay; Non-structural protein | Issue Date: | 2013 | Journal Volume: | 396 | Journal Issue: | 1-2 | Start page/Pages: | 87-95 | Source: | Journal of Immunological Methods | Abstract: | Foot-and mouth disease (FMD), swine vesicular disease (SVD), and vesicular stomatitis (VS) are highly contagious vesicular diseases of swine but are not easy to differentiate clinically. For the purpose of instant detecting of FMD and differentiating it from the other vesicular diseases, a Luminex assay was developed. Sera from 64 infected, 307 vaccinated, and 280 na?ve pigs were tested by the Luminex assay. Diagnostic sensitivity of the assay was 100%. Diagnostic specificity of the assay was 98.7% in vaccinated pigs and 97.5% to 100% in na?ve pigs. Agreement between the results from the Luminex assay and those from a 3ABC polypeptide blocking ELISA was 96.3% with kappa statistics of 0.92. The Luminex assay can detect the immune response to NSP-3ABC in swine as early as eight days post-infection. Moreover, all of the 15 vaccinated but unprotected pigs were all detected by the Luminex assay. The results indicated that the Luminex assay has potential with specificity in detecting antibodies to FMDV 3ABC NSP and in distinguishing FMDV-infected pigs from with either SVDV or VSV. ? 2013 Elsevier B.V. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84884354339&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/380243 |
DOI: | 10.1016/j.jim.2013.08.002 | metadata.dc.subject.other: | nonstructural protein 3ABC antibody; protein antibody; unclassified drug; virus antibody; virus vaccine; 3ABC blocking ELISA kit; antibody blood level; antibody detection; article; chromatography; controlled clinical trial; controlled study; diagnostic test accuracy study; ELISA kit; enzyme linked immunosorbent assay; foot and mouth disease; Foot and mouth disease virus; immune response; immunoassay; Luminex assay; nonhuman; nucleotide sequence; priority journal; sensitivity and specificity; swine; vaccination; virus neutralization; Antibody detection; days post infection; dpi; ELISA; Enzyme-linked immunosorbent assay; enzyme-linked immunosorbent assay; FMD; FMDV; foot-and-mouth disease; foot-and-mouth disease virus; Foot-and-mouth disease virus; horseradish peroxidase; HRP; Luminex assay; median fluorescent intensity; MFI; multi-analyte profiling; NC; negative control; non-structural protein; Non-structural protein; NSP; OD; optical density; PC; percentage of inhibition; PI; positive control; SP; specific-pathogen-free; SPF; structural protein; SVDV; swine vesicular disease virus; T/C; test to control; vesicular stomatitis virus; VSV; xMAP; Animals; Antibodies, Viral; Diagnosis, Differential; Foot-and-Mouth Disease; Foot-and-Mouth Disease Virus; Immunoassay; Microspheres; Sensitivity and Specificity; Swine; Swine Diseases; Swine Vesicular Disease; Vaccination; Vesicular Stomatitis; Viral Nonstructural Proteins; Viral Vaccines; Foot-and-mouth disease virus; Suidae; Vesicular stomatitis virus [SDGs]SDG3 |
| Appears in Collections: | 獸醫學系 |
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