https://scholars.lib.ntu.edu.tw/handle/123456789/384327
標題: | Activation of antitumor immune responses by Ganoderma formosanum polysaccharides in tumor-bearing mice | 作者: | Wang, Cheng-Li Lu, Chiu-Ying Hsueh, Ying-Chao Liu, Wen-Hsiung Chen, Chun-Jen CHUN-JEN CHEN |
關鍵字: | Antitumor; Ganoderma formosanum; Immunomodulation; Polysaccharides | 公開日期: | 2014 | 卷: | 98 | 期: | 22 | 起(迄)頁: | 9389-9398 | 來源出版物: | Applied Microbiology and Biotechnology | 摘要: | Fungi of the genus Ganoderma are basidiomycetes that have been used as traditional medicine in Asia and have been shown to exhibit various pharmacological activities. We recently found that PS-F2, a polysaccharide fraction purified from the submerged culture broth of Ganoderma formosanum, stimulates the maturation of dendritic cells and primes a T helper 1 (Th1)-polarized adaptive immune response in vivo. In this study, we investigated whether the immune adjuvant function of PS-F2 can stimulate antitumor immune responses in tumor-bearing mice. Continuous intraperitoneal or oral administration of PS-F2 effectively suppressed the growth of colon 26 (C26) adenocarcinoma, B16 melanoma, and sarcoma 180 (S180) tumor cells in mice without adverse effects on the animals’ health. PS-F2 did not cause direct cytotoxicity on tumor cells, and it lost the antitumor effect in mice with severe combined immunodeficiency (SCID). CD4+ T cells, CD8+ T cells, and serum from PS-F2-treated tumor-bearing mice all exhibited antitumor activities when adoptively transferred to na?ve animals, indicating that PS-F2 treatment stimulates tumor-specific cellular and humoral immune responses. These data demonstrate that continuous administration of G. formosanum polysaccharide PS-F2 can activate host immune responses against ongoing tumor growth, suggesting that PS-F2 can potentially be developed into a preventive/therapeutic agent for cancer immunotherapy. ? 2014, Springer-Verlag Berlin Heidelberg. |
URI: | http://scholars.lib.ntu.edu.tw/handle/123456789/384327 | DOI: | 10.1007/s00253-014-6027-6 | SDG/關鍵字: | Cytology; Fungi; Immune system; Mammals; Polysaccharides; Tumors; Adaptive immune response; Anti-tumor activities; Anti-tumors; Ganoderma; Humoral immune response; Immuno modulations; Pharmacological activity; Severe combined immunodeficiencies; T-cells; antineoplastic agent; fungal polysaccharide; immunological adjuvant; ps f2; unclassified drug; fungal polysaccharide; immunologic factor; bioassay; cancer; fungus; immune response; medicine; polysaccharide; rodent; toxicity; tumor; adenocarcinoma cell line; adoptive transfer; animal cell; animal experiment; animal model; antineoplastic activity; Article; cancer immunotherapy; cancer inhibition; cancer prevention; CD4+ T lymphocyte; CD8+ T lymphocyte; cell maturation; cellular immunity; continuous infusion; controlled study; dendritic cell; drug cytotoxicity; experimental neoplasm; experimental sarcoma; fungus culture; Ganoderma; Ganoderma formosanum; humoral immunity; immunoreactivity; in vivo study; male; melanoma B16; melanoma cell line; mouse; nonhuman; sarcoma 180; sarcoma cell line; severe combined immunodeficiency; T lymphocyte activation; Th1 cell; treatment indication; animal; disease model; Ganoderma; immunotherapy; isolation and purification; metabolism; Neoplasms; procedures; treatment outcome; Asia; Animalia; Basidiomycota; Fungi; Ganoderma; Ganoderma formosanum; Mus; Animals; Disease Models, Animal; Fungal Polysaccharides; Ganoderma; Immunologic Factors; Immunotherapy; Mice; Neoplasms; Treatment Outcome |
顯示於: | 生化科技學系 |
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