https://scholars.lib.ntu.edu.tw/handle/123456789/384335
標題: | Amniotic fluid stem cells prevent follicle atresia and rescue fertility of mice with premature ovarian failure induced by chemotherapy | 作者: | Xiao, G.-Y. I-HSUAN LIU Cheng, C.-C. Chang, C.-C. Lee, Y.-H. Cheng, W.T.-K. SHINN-CHIH WU |
公開日期: | 2014 | 卷: | 9 | 期: | 9 | 來源出版物: | PloS one | 摘要: | Chemotherapy used to treat cancer may cause irreversible premature ovarian failure (POF). Of late, amniotic fluid stem cells (AFSCs) provide a novel source for regenerative medicine because of their primitive stage, low immunogenicity, and easy accessibility. In this study, we isolated AFSCs from transgenic mice that ubiquitously express enhanced green fluorescence protein (EGFP). These AFSCs exhibited morphologies, immunophenotypes, and mesoderm trilineage differentiation potentials similar to mesenchymal stem cells (MSCs). Further, AFSCs proliferated faster than MSCs and expressed OCT4, a marker for pluripotency. To investigate their potential in recovering fertility in POF model, AFSCs were transplanted into the ovaries of mice with POF six weeks post induction using chemotherapeutic drugs, busulfan and cyclophosphamide. AFSCs could rescue the reproductive ability of mice with POF by preventing follicle atresia and sustaining the healthy follicles. Notably, the transplanted AFSCs did not differentiate into granulosa and germline cells in vivo. After one month, the decreased numbers of transplanted AFSCs accompanied with the reduced beneficial effects indicated that the therapeutic efficacy were directly from AFSCs. These findings demonstrated the therapeutic effects of AFSCs and suggested the promise of AFSCs for treating infertility and POF caused by chemotherapy. ? 2014 Xiao et al. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84929940124&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/384335 |
DOI: | 10.1371/journal.pone.0106538 | SDG/關鍵字: | antineoplastic agent; busulfan; cyclophosphamide; enhanced green fluorescent protein; octamer transcription factor 4; adipogenesis; amnion fluid; animal experiment; animal model; Article; cell differentiation; cell proliferation; controlled study; drug efficacy; female; flow cytometry; granulosa cell; immunocytochemistry; immunophenotyping; in vivo study; mesenchymal stem cell; mesoderm; mouse; nonhuman; ovary follicle atresia; ovary function; premature ovarian failure; protein expression; stem cell; transgenic mouse; vagina cytology; Mus |
顯示於: | 動物科學技術學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。