|Title:||Enhanced amelioration of high-fat diet-induced fatty liver by docosahexaenoic acid and lysine supplementations||Authors:||Lin, H.-Y.
|Issue Date:||2014||Journal Volume:||2014||Source:||BioMed Research International||Abstract:||
Fatty liver disease is the most common pathological condition in the liver. Here, we generated high-fat diet-(HFD-) induced nonalcoholic fatty liver disease (NAFLD) in mice and tested the effects of docosahexaenoic acid (DHA) and lysine during a four-week regular chow (RC)feeding. Our results showed that 1% lysine and the combination of 1% lysine + 1% DHA reduced body weight. Moreover, serum triglyceride levels were reduced by 1% DHA and 1% lysine, whereas serum alanine transaminase activity was reduced by 1% DHA and 1% DHA + 0.5% lysine. Switching to RC reduced hepatic lipid droplet accumulation, which was further reduced by the addition of DHA or lysine. Furthermore, the mRNA expressions of hepatic proinflammatory cytokines were suppressed by DHA and combinations of DHA + lysine, whereas the mRNA for the lipogenic gene, acetyl-CoA carboxylase 1 (ACC1), was suppressed by DHA. In the gonadal adipose tissues, combinations of DHA and lysine inhibited mRNA expression of lipid metabolism-associated genes, including ACC1, fatty acid synthase, lipoprotein lipase, and perilipin. In conclusion, the present study demonstrated that, in conjunction with RC-induced benefits, supplementation with DHA or lysine further ameliorated the high-fat diet-induced NAFLD and provided an alternative strategy to treat, and potentially prevent, NAFLD. ? 2014 Hsin-Yu Lin et al.
|DOI:||10.1155/2014/310981||SDG/Keyword:||acetyl coenzyme A carboxylase; acetyl coenzyme A carboxylase 1; alanine aminotransferase; cytokine; docosahexaenoic acid; fatty acid synthase; lipoprotein lipase; lysine; perilipin; triacylglycerol; unclassified drug; docosahexaenoic acid; fat intake; lysine; animal experiment; animal model; animal tissue; article; body weight; controlled study; human; human cell; lipid diet; lipid liver level; lipid metabolism; male; mouse; nonalcoholic fatty liver; nonhuman; nucleotide sequence; treatment duration; triacylglycerol blood level; animal; chemically induced; diet supplementation; diet therapy; drug effects; fat intake; fatty liver; gene expression regulation; liver; metabolism; Animals; Dietary Fats; Dietary Supplements; Docosahexaenoic Acids; Fatty Liver; Gene Expression Regulation; Lipid Metabolism; Liver; Lysine; Mice
|Appears in Collections:||動物科學技術學系|
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