|Title:||Evaluating the Bone Tissue Regeneration Capability of the Chinese Herbal Decoction Danggui Buxue Tang from a Molecular Biology Perspective||Authors:||Wang, W.-L.
|Issue Date:||2014||Journal Volume:||2014||Source:||BioMed Research International||Abstract:||
Large bone defects are a considerable challenge to reconstructive surgeons. Numerous traditional Chinese herbal medicines have been used to repair and regenerate bone tissue. This study investigated the bone regeneration potential of Danggui Buxue Tang (DBT), a Chinese herbal decoction prepared from Radix Astragali (RA) and Radix Angelicae Sinensis (RAS), from a molecular biology perspective. The optimal ratio of RA and RAS used in DBT for osteoblast culture was obtained by colorimetric and alkaline phosphatase (ALP) activity assays. Moreover, the optimal concentration of DBT for bone cell culture was also determined by colorimetric, ALP activity, nodule formation, Western blotting, wound-healing, and tartrate-resistant acid phosphatase activity assays. Consequently, the most appropriate weight ratio of RA to RAS for the proliferation and differentiation of osteoblasts was 5: 1. Moreover, the most effective concentration of DBT was 1,000 μg/mL, which significantly increased the number of osteoblasts, intracellular ALP levels, and nodule numbers, while inhibiting osteoclast activity. Additionally, 1,000 g/mL of DBT was able to stimulate p-ERK and p-JNK signal pathway. Therefore, DBT is highly promising for use in accelerating fracture healing in the middle or late healing periods. ? 2014 Wen-Ling Wang et al.
|DOI:||10.1155/2014/853234||SDG/Keyword:||acid phosphatase tartrate resistant isoenzyme; alkaline phosphatase; bone density conservation agent; danggui buxue tang; herbaceous agent; mitogen activated protein kinase; stress activated protein kinase; unclassified drug; wound healing promoting agent; angelicae sinensis extract; danggui buxue decoction; herbaceous agent; Huang Qi; animal cell; Article; bone regeneration; bone tissue; cell differentiation; cell proliferation; Chinese medicine; concentration response; controlled study; drug efficacy; effective concentration; ethnopharmacology; herbal medicine; human; human cell; intracellular signaling; molecular biology; molecular weight; mouse; nonhuman; osteoblast; osteoclast activity; osteoclastogenesis; Western blotting; wound healing; bone regeneration; chemistry; drug effects; metabolism; signal transduction; Bone Regeneration; Drugs, Chinese Herbal; Humans; MAP Kinase Signaling System; Osteoblasts
|Appears in Collections:||獸醫學系|
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