Neutrophil gelatinase-associated lipocalin in dogs with naturally occurring renal diseases
Journal
Journal of Veterinary Internal Medicine
Journal Volume
28
Journal Issue
2
Pages
437-442
Date Issued
2014
Author(s)
Abstract
Background: Neutrophil gelatinase-associated lipocalin (NGAL) is released from renal tubular cells after injury and serves in humans as a real-time indicator of active kidney damage, including acute kidney injury (AKI) and chronic kidney disease (CKD). However, NGAL concentrations in dogs with naturally occurring AKI or CKD rarely have been explored in detail. Hypothesis/Objectives: The goal of this study was to evaluate whether NGAL can serve as a useful biomarker in dogs with naturally occurring renal disease. Animals: Client-owned dogs with renal disease (57) and control dogs without any disease (12) were examined. Methods: Serum NGAL (sNGAL) and urine NGAL (uNGAL) concentrations were measured in each animal by a newly developed ELISA system. Demographic, hematologic, and serum biochemical data were recorded. Survival attributable to AKI and CKD was evaluated at 30 days and 90 days, respectively. Results: Serum and urine NGAL concentrations in azotemic dogs were significantly higher than in nonazotemic dogs and were highly correlated with serum creatinine concentration (P < .05). Among CKD dogs, death was associated with significantly higher sNGAL and uNGAL concentrations compared with survivors. Receiver-operating characteristic curve (ROC) analysis showed that sNGAL was better than serum creatinine concentration when predicting clinical outcomes for CKD dogs (P < .05). The best cutoff point for sNGAL was 50.6 ng/mL, which gave a sensitivity and a specificity of 76.9 and 100%, respectively. Furthermore, dogs that had higher concentrations of sNGAL survived for a significantly shorter time. Conclusion: sNGAL is a useful prognostic marker when evaluating dogs with CKD. ? 2014 by the American College of Veterinary Internal Medicine.
Subjects
Azotemia; Outcome; Renal biomarkers; Renal failure; Survival
SDGs
Other Subjects
Animalia; Canis familiaris; acute phase protein; biological marker; lipocalin; neutrophil gelatinase-associated lipocalin protein, dog; oncoprotein; Acute Kidney Injury; animal; blood; case control study; dog; Dog Diseases; enzyme linked immunosorbent assay; female; Kidney Diseases; male; prognosis; Renal Insufficiency, Chronic; veterinary; Acute Kidney Injury; Acute-Phase Proteins; Animals; Biological Markers; Case-Control Studies; Dog Diseases; Dogs; Enzyme-Linked Immunosorbent Assay; Female; Kidney Diseases; Lipocalins; Male; Prognosis; Proto-Oncogene Proteins; Renal Insufficiency, Chronic
Type
journal article