https://scholars.lib.ntu.edu.tw/handle/123456789/385764
標題: | Two azole fungicides (carcinogenic triadimefon and non-carcinogenic myclobutanil) exhibit different hepatic cytochrome P450 activities in medaka fish | 作者: | Lin, C.-H. Chou, P.-H. PEI-JEN CHEN |
關鍵字: | Conazoles; Cytochrome P450 enzymes (CYPs); Medaka (Oryzias latipes); Myclobutanil; Triadimefon | 公開日期: | 2014 | 卷: | 277 | 起(迄)頁: | 150-158 | 來源出版物: | Journal of Hazardous Materials | 摘要: | Conazoles are a class of imidazole- or triazole-containing drugs commonly used as fungicides in agriculture and medicine. The broad application of azole drugs has led to the contamination of surface aquifers receiving the effluent of municipal or hospital wastewater or agricultural runoff. Several triazoles are rodent carcinogens; azole pollution is a concern to environmental safety and human health. However, the carcinogenic mechanisms associated with cytochrome P450 enzymes (CYPs) of conazoles remain unclear. We exposed adult medaka fish (Oryzias latipes) to continuous aqueous solutions of carcinogenic triadimefon and non-carcinogenic myclobutanil for 7 to 20 days at sub-lethal or environmentally relevant concentrations and assessed hepatic CYP activity and gene expression associated with CYP-mediated toxicity. Both triadimefon and myclobutanil induced hepatic CYP3A activity, but only triadimefon enhanced CYP1A activity. The gene expression of cyp3a38, cyp3a40, pregnane x receptor (pxr), cyp26b, retinoid acid receptor γ1 (rarγ1) and p53 was higher with triadimefon than myclobutanil. As well, yeast-based reporter gene assay revealed that 4 tested conazoles were weak agonists of aryl hydrocarbon receptor (AhR). We reveal differential CYP gene expression with carcinogenic and non-carcinogenic conazoles in a lower vertebrate, medaka fish. Liver CYP-enzyme induction may be a key event in conazole-induced tumorigenesis. This information is essential to evaluate the potential threat of conazoles to human health and fish populations in the aquatic environment. © 2014 Elsevier B.V. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84903733134&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/385764 |
DOI: | 10.1016/j.jhazmat.2014.05.083 | SDG/關鍵字: | Aquifers; Effluents; Enzymes; Fish; Fisheries; Fungicides; Gene expression; Health risks; Hydrogeology; Living systems studies; Plants (botany); Conazoles; Cytochrome p450 enzymes; Myclobutanil; Oryzias latipes; Triadimefon; Groundwater pollution; aromatic hydrocarbon receptor; cell receptor; cytochrome P450; cytochrome P450 1A; cytochrome P450 3A; ketoconazole; myclobutanil; pregnane X receptor; propiconazole; protein p53; retinoid acid receptor gamma 1; triadimefon; triadimenol; unclassified drug; cytochrome P450; fungicide; nitrile; systhane; triadimefon; triazole derivative; water pollutant; aqueous solution; bioaccumulation; carcinogen; cytochrome; drug; ecotoxicology; enzyme activity; fungicide; gene expression; public health; teleost; tumor; adult; animal cell; animal experiment; animal model; animal tissue; aquatic environment; article; controlled study; enzyme activity; enzyme induction; gene expression; liver microsome; male; nonhuman; Oryzias; oxidative stress; pollution; reporter gene; vertebrate; waste water; yeast; animal; drug effects; enzymology; liver; metabolism; real time polymerase chain reaction; toxicity; water pollutant; Oryzias latipes; Oryziinae; Rodentia; Vertebrata; Animals; Cytochrome P-450 Enzyme System; Fungicides, Industrial; Liver; Microsomes, Liver; Nitriles; Oryzias; Real-Time Polymerase Chain Reaction; Triazoles; Water Pollutants, Chemical |
顯示於: | 農業化學系 |
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