|Title:||A complete molecular diagnostic procedure for applications in surveillance and subtyping of avian influenza virus||Authors:||Tseng, C.-H.
|Issue Date:||2014||Journal Volume:||2014||Source:||BioMed Research International||Abstract:||
Introduction. The following complete molecular diagnostic procedure we developed, based on real-time quantitative PCR and traditional PCR, is effective for avian influenza surveillance, virus subtyping, and viral genome sequencing. Method. This study provides a specific and sensitive step-by-step procedure for efficient avian influenza identification of 16 hemagglutinin and 9 neuraminidase avian influenza subtypes. Result and Conclusion. This diagnostic procedure may prove exceedingly useful for virological and ecological advancements in global avian influenza research. ? 2014 Chun-Hsien Tseng et al.
|DOI:||10.1155/2014/653056||SDG/Keyword:||hemagglutinin; sialidase; hemagglutinin, avian influenza A virus; Influenza virus hemagglutinin; organic compound; sialidase; SYBR Green I; article; avian influenza; avian influenza virus; diagnostic procedure; health survey; human; molecular diagnosis; nonhuman; nucleotide sequence; real time polymerase chain reaction; sequence analysis; virus strain; animal; avian influenza; bird; chemistry; classification; DNA sequence; genetics; molecular pathology; polymerase chain reaction; procedures; reverse transcription polymerase chain reaction; virology; virus genome; Animals; Birds; Genome, Viral; Hemagglutinin Glycoproteins, Influenza Virus; Influenza in Birds; Neuraminidase; Organic Chemicals; Pathology, Molecular; Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA
|Appears in Collections:||獸醫學系|
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