https://scholars.lib.ntu.edu.tw/handle/123456789/388484
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Hui, Y. Y. | en_US |
dc.contributor.author | Su, L. J. | en_US |
dc.contributor.author | Chen, O. Y. | en_US |
dc.contributor.author | Chen, Y. T. | en_US |
dc.contributor.author | Liu, T. M. | en_US |
dc.contributor.author | Chang, H. C. | en_US |
dc.contributor.author | YIT-TSONG CHEN | zz |
dc.creator | Hui, Y. Y.; Su, L. J.; Chen, O. Y.; Chen, Y. T.; Liu, T. M.; Chang, H. C. | - |
dc.date.accessioned | 2018-09-10T15:00:06Z | - |
dc.date.available | 2018-09-10T15:00:06Z | - |
dc.date.issued | 2014 | - |
dc.identifier.uri | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000338506300002&KeyUID=WOS:000338506300002 | - |
dc.identifier.uri | http://scholars.lib.ntu.edu.tw/handle/123456789/388484 | - |
dc.description.abstract | Nanodiamonds containing high density ensembles of negatively charged nitrogen-vacancy (NV-) centers are promising fluorescent biomarkers due to their excellent photostability and biocompatibility. The NV-?' centers in the particles have a fluorescence lifetime of up to 20ns ...ns, which distinctly differs from those (<10 ...ns) of cell and tissue autofluorescence, making it possible to achieve background-free detection in vivo by time gating. Here, we demonstrate the feasibility of using fluorescent nanodiamonds (FNDs) as optical labels for wide-field time-gated fluorescence imaging and flow cytometric analysis of cancer cells with a nanosecond intensified charge-coupled device (ICCD) as the detector. The combined technique has allowed us to acquire fluorescence images of FND-labeled HeLa cells in whole blood covered with a chicken breast of ?0.1-mm thickness at the single cell level, and to detect individual FND-labeled HeLa cells in blood flowing through a microfluidic device at a frame rate of 23 ...Hz, as well as to locate and trace FND-labeled lung cancer cells in the blood vessels of a mouse ear. It opens a new window for real-time imaging and tracking of transplanted cells (such as stem cells) in vivo. | - |
dc.language | en | en |
dc.relation.ispartof | Scientific Reports | en_US |
dc.source | AH-ResearcherID | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | fluorescent dye; nanodiamond; animal; Bagg albino mouse; cancer transplantation; cell tracking; chemistry; chicken; flow cytometry; fluorescence microscopy; HeLa cell line; human; metabolism; microfluidic analysis; tumor embolism; Animals; Cell Tracking; Chickens; Flow Cytometry; Fluorescent Dyes; HeLa Cells; Humans; Mice, Inbred BALB C; Microfluidic Analytical Techniques; Microscopy, Fluorescence; Nanodiamonds; Neoplasm Transplantation; Neoplastic Cells, Circulating | - |
dc.title | Wide-field imaging and flow cytometric analysis of cancer cells in blood by fluorescent nanodiamond labeling and time gating | - |
dc.type | journal article | en |
dc.identifier.doi | 10.1038/srep05574 | - |
dc.identifier.scopus | 2-s2.0-84903974191 | - |
dc.identifier.isi | WOS:000338506300002 | - |
dc.relation.pages | 7 | - |
dc.relation.journalvolume | 4 | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Chemistry | - |
crisitem.author.orcid | 0000-0002-6204-8320 | - |
crisitem.author.parentorg | College of Science | - |
顯示於: | 化學系 |
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