https://scholars.lib.ntu.edu.tw/handle/123456789/38861
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Lin, Yu-Shih | en |
dc.contributor.author | Lipp, Julius S. | en |
dc.contributor.author | Yoshinaga, Marcos Y. | en |
dc.contributor.author | Lin, Shao-Hsuan | en |
dc.contributor.author | Elvert, Marcus | en |
dc.contributor.author | Hinrichs, Kai-Uwe | en |
dc.creator | Lin, Yu-Shih;Lipp, Julius S.;Yoshinaga, Marcos Y.;Lin, Shao-Hsuan;Elvert, Marcus;Hinrichs, Kai-Uwe | en |
dc.date | 2010 | en |
dc.date.accessioned | 2012-07-02T13:16:57Z | - |
dc.date.accessioned | 2018-06-28T13:12:53Z | - |
dc.date.available | 2012-07-02T13:16:57Z | - |
dc.date.available | 2018-06-28T13:12:53Z | - |
dc.date.issued | 2010 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/240564 | - |
dc.description.abstract | Glycolipids are prominent constituents in the membranes of cells from all domains of life. For example, diglycosyl-glycerol dibiphytanyl glycerol tetraethers (2Gly-GDGTs) are associated with methanotrophic ANME-1 archaea and heterotrophic benthic archaea, two archaeal groups of global biogeochemical importance. The hydrophobic biphytane moieties of 2Gly-GDGTs from these two uncultivated archaeal groups exhibit distinct carbon isotopic compositions. To explore whether the isotopic compositions of the sugar headgroups provide additional information on the metabolism of their producers, we developed a procedure to analyze the δ13C values of glycosidic headgroups. Successful determination was achieved by (1) monitoring the contamination from free sugars during lipid extraction and preparation, (2) optimizing the hydrolytic conditions for glycolipids, and (3) derivatizing the resulting sugars into aldononitrile acetate derivatives, which are stable enough to withstand a subsequent column purification step. First results of δ13C values of sugars cleaved from 2Gly-GDGTs in two marine sediment samples, one containing predominantly ANME-1 archaea and the other benthic archaea, were obtained and compared with the δ13C values of the corresponding biphytanes. In both samples the dominant sugar headgroups were enriched in 13C relative to the corresponding major biphytane. This 13C enrichment was significantly larger in the putative major glycolipids from ANME-1 archaea (~15‰) than in those from benthic archaea (<7‰). This method opens a new analytical window for the examination of carbon isotopic relationships between sugars and lipids in uncultivated organisms. © 2010 John Wiley & Sons, Ltd. | - |
dc.language | en | en |
dc.language.iso | en_US | - |
dc.relation | Rapid Communications in Mass Spectrometry, 24(19), 2817-2826 | en |
dc.relation.ispartof | Rapid Communications in Mass Spectrometry | en_US |
dc.subject.classification | [SDGs]SDG14 | - |
dc.subject.other | carbon; diacylglycerol; glycolipid; membrane lipid; archaebacterium; article; chemistry; isolation and purification; isotope labeling; methodology; sediment; Archaea; Carbon Isotopes; Diglycerides; Geologic Sediments; Glycolipids; Isotope Labeling; Membrane Lipids | - |
dc.title | Intramolecular stable carbon isotopic analysis of archaeal glycosyl tetraether lipids | en |
dc.type | journal article | en |
dc.identifier.doi | 10.1002/rcm.4707 | en |
dc.relation.pages | 2817-2826 | - |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/240564/-1/162.pdf | - |
item.openairetype | journal article | - |
item.fulltext | with fulltext | - |
item.languageiso639-1 | en_US | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
顯示於: | 地質科學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。