https://scholars.lib.ntu.edu.tw/handle/123456789/390094
Title: | Supplementation of Chitosan Alleviates High-Fat Diet-Enhanced Lipogenesis in Rats via Adenosine Monophosphate (AMP)-Activated Protein Kinase Activation and Inhibition of Lipogenesis-Associated Genes | Authors: | Chiu, Chen-Yuan SHING-HWA LIU Chan, Im-Lam Yang, Tsung-Han Liu, Shing-Hwa Chiang, Meng-Tsan |
Keywords: | AMP-activated protein kinase; chitosan; gene expression; high-fat diet; lipogenesis | Issue Date: | 2015 | Journal Volume: | 63 | Journal Issue: | 11 | Start page/Pages: | 2979-2988 | Source: | Journal of Agricultural and Food Chemistry | Abstract: | This study investigated the role of chitosan in lipogenesis in high-fat diet-induced obese rats. The lipogenesis-associated genes and their upstream regulatory proteins were explored. Diet supplementation of chitosan efficiently decreased the increased weights in body, livers, and adipose tissues in high-fat diet-fed rats. Chitosan supplementation significantly raised the lipolysis rate; attenuated the adipocyte hypertrophy, triglyceride accumulation, and lipoprotein lipase activity in epididymal adipose tissues; and decreased hepatic enzyme activities of lipid biosynthesis. Chitosan supplementation significantly activated adenosine monophosphate (AMP)-activated protein kinase (AMPK) phosphorylation and attenuated high-fat diet-induced protein expressions of lipogenic transcription factors (PPAR-γ and SREBP1c) in livers and adipose tissues. Moreover, chitosan supplementation significantly inhibited the expressions of downstream lipogenic genes (FAS, HMGCR, FATP1, and FABP4) in livers and adipose tissues of high-fat diet-fed rats. These results demonstrate for the first time that chitosan supplementation alleviates high-fat diet-enhanced lipogenesis in rats via AMPK activation and lipogenesis-associated gene inhibition. ? 2015 American Chemical Society. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84926156171&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/390094 |
DOI: | 10.1021/acs.jafc.5b00198 | SDG/Keyword: | Biochemistry; Chemical activation; Chitin; Chitosan; Enzyme activity; Enzymes; Genes; Histology; Nutrition; Proteins; Rats; Tissue; Transcription; Activated proteins; Adenosine monophosphate; Adipocyte hypertrophy; AMP-activated protein kinase; High-fat diet; Lipogenesis; Lipoprotein lipase; Regulatory protein; Gene expression; Rattus; chitosan; FABP4 protein, rat; fatty acid binding protein; hydroxymethylglutaryl coenzyme A reductase kinase; peroxisome proliferator activated receptor gamma; sterol regulatory element binding protein 1; adipose tissue; adverse effects; analysis; animal; diet supplementation; drug effects; enzyme activation; enzymology; gene expression; genetics; human; lipid diet; lipogenesis; liver; male; metabolism; obesity; pathophysiology; rat; Sprague Dawley rat; Adipose Tissue; AMP-Activated Protein Kinases; Animals; Chitosan; Diet, High-Fat; Dietary Supplements; Enzyme Activation; Fatty Acid-Binding Proteins; Gene Expression; Humans; Lipogenesis; Liver; Male; Obesity; PPAR gamma; Rats; Rats, Sprague-Dawley; Sterol Regulatory Element Binding Protein 1 |
Appears in Collections: | 毒理學研究所 |
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